catalog number :
MBS142453
products type :
Recombinant Protein
products full name :
Recombinant Urate Oxidase
products short name :
Urate Oxidase
products name syn :
Uricase; Urate Oxidase Recombinant; Urate Oxidase; Uricase; Urate Oxygen; Oxidoreductase; UOX; UO; EC 1.7.3; Uricase
other names :
uricase; Uricase; uricase; urate oxidase 2; urate oxidase; Urate oxidase
other gene names :
Uox; Uox; Uri; Uri2; UOX-2
uniprot entry name :
URIC_RAT
sequence :
msavkaaryg kdnvrvykvh kdektgvqtv yemtvcvlle geietsytka dnsvivatds ikntiyitak qnpvtppelf gsilgthfie kynhihaahv nivchrwtrm didgkphphs firdseekrn vqvdvvegkg idiksslsgl tvlkstnsqf wgflrdeytt lketwdrils tdvdatwqwk nfsglqevrs hvpkfdatwa tarevtlktf aednsasvqa tmykmaeqil arqqlietve yslpnkhyfe idlswhkglq ntgknaevfa pqsdpnglik ctvgrsslks kl.
purity :
Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Each 1.5mg Uricase contains 5mg sucrose, 25mg glycine, 0.1mg Tween-80, 13.6 mg Na2HPO4*12H20 and 0.33 mg NaH2PO4*2H20. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Urate Oxidase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Uricase should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
other info2 :
Solubility: We highly recommend reconstituting the lyophilized Uricase in 50mM borate buffer containing 0.001%Triton X-100 and 1.0mM EDTA, pH 8.5 for activity assay. Biological Activity: The specific activity was found to be 10U/mg.One Unit oxidizes one micromole of uric acid per minute at 25 degree C, at pH 8.5.
products categories :
ENZYMES; Enzymes; Oxidase
products description :
Description: Urate Oxidase Recombinant produced in E Coli is a tetrameric, non-glycosylated polypeptide chain containing 302 amino acids, having a molecular formula of C1523H2383N417O462S7 and a molecular mass of 34,247 Dalton.The cDNA coding for urate-oxidase was cloned from a strain of Aspergillus flavus. The monomer protein has no intra- or inter-disulfide bridges.
ncbi acc num :
NP_446220.1
ncbi gb acc num :
NM_053768.2
ncbi mol weight :
34,934 Da
ncbi pathways :
Caffeine Metabolism Pathway (83338); Caffeine Metabolism Pathway (308); Metabolic Pathways (132933); Purine Metabolism Pathway (83337); Purine Metabolism Pathway (307)
ncbi summary :
catalyzes the hydrolysis of uric acid to allantoin in purine degradation [RGD, Feb 2006]
uniprot summary :
uricase: Catalyzes the oxidation of uric acid to 5- hydroxyisourate, which is further processed to form (S)-allantoin. Belongs to the uricase family. Protein type: EC 1.7.3.3; Oxidoreductase; Mitochondrial. Cellular Component: peroxisomal matrix; mitochondrion; peroxisome. Molecular Function: urate oxidase activity. Biological Process: urate catabolic process; purine base catabolic process