product summary
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company name :
MyBioSource
product type :
protein
product name :
Recombinant Taq Plus DNA Polymerase
catalog :
MBS142450
quantity :
250 Units
price :
140 USD
more info or order :
product information
catalog number :
MBS142450
products type :
Recombinant Protein
products full name :
Recombinant Taq Plus DNA Polymerase
products short name :
Taq Plus DNA Polymerase
products name syn :
Taq Plus DNA; Taq Plus DNA Polymerase Recombinant; Taq Plus DNA Polymerase; Taq Plus DNA; TaqPDNA; Taq Plus DNA
host :
Recombinant E Coli contains Thermus aquaticus polymerase gene
concentration :
5U/ ul.
storage stability :
Stable for 5 days at 10 degree C, for longer period of time store at -20 degree C.
other info1 :
10X Reaction Buffer: 200 mM TrisHCI (pH 8.8)100 mM KCI100 mM (NH4)2SO420 mM Mg SO41% Triton X-1001 mg/ml bovine serum albumin (BSA). Optimization of DNA Synthesis: It is important to ad the reaction components in the following order1. H2O.2. 10 x reaction buffer.3. dNTPs.4. DNA template and primers.5. Taq Plus. Reaction Conditions: DNA synthesis is performed in 100ml of mixture containing 20-200um dNTPs, 0.3-1um Promers, 0.1-0.250 mg of template DNA, 10 ?l of 10 x reaction buffer and 2.5-5 units of Taq Plus. Mix the reaction gently, centrifuge briefly and then overlay with light mineral oil. Initially, denature the reaction by incubating at 95? for 5 minutes and then cool to 40-68? for 5 minutes to allow the primers to anneal to the template DNA. Unit Defenition: One unit of the enzyme catalyzes the incorporation of 10nmole of deoxyribonucleotides into a polynucleotide fraction in 30 min at 74 degree C. Storage Buffer: 20mM Tris-HCl (pH 8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, Stabilizers, and 50%glycerol.
other info2 :
Note: All reagents, including Taq Plus, should be mixed immediately before use.
products categories :
ENZYMES; Enzymes; DNA Polymerase
products description :
Description: Taq Plus is a mixture of Taq and Pfu. Taq Plus, which is used to improve the reliability and yield of conventional primer extension reaction. Taq Plus has two following advantages over Taq: (1) high fidelity with an error frequency 1.6/106 (or 0.0016/103) during DNA synthesis. (2) Taq Plus increases the efficiency of polymerization reaction, resulting in a great percentage of extenuation reaction completion up to 10 kb to 30 kb. Pfu has a temperature optimum between 72-78 degree C and remains > 95% active following 1-hour incubation at 95 degree C.
size1 :
250 Units
price1 :
140 USD
size2 :
750 Units
price2 :
205
size3 :
2500 Units
price3 :
415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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