product summary
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company name :
MyBioSource
product type :
protein
product name :
Recombinant Taq DNA Polymerase
catalog :
MBS142449
quantity :
1000 Units
price :
140 USD
more info or order :
product information
catalog number :
MBS142449
products type :
Recombinant Protein
products full name :
Recombinant Taq DNA Polymerase
products short name :
Taq DNA Polymerase
products name syn :
TaqDNA; Taq DNA Polymerase Recombinant; DNA polymerase I thermostable; EC 2.7.7.7; Taq polymerase 1; TaqDNA
other names :
DNA polymerase I, thermostable; DNA polymerase I, thermostable; Taq polymerase 1
other gene names :
polA; pol1
uniprot entry name :
DPO1_THEAQ
host :
Recombinant E Coli contains Thermus aquaticus polymerase gene
sequence length :
832
form :
Taq DNA Polymerase solution in 20mM Tris-HCl, pH 8.0, 100mM KCl, 0.1mM EDTA, 5mM DTT, 50% Glycerol, 0.5% NP40, 0.5% Tween 20.
storage stability :
Stable for 5 days at 10 degree C, for longer period of time store at -20 degree C.
other info1 :
Include: : 10X Reaction Buffer without MgCl2 and separate 25mM MgCl2 Solution. Features: Depend on an Enzyme That Works: Compositions of the storage buffers have been optimized to assure quality performance of the enzyme under a variety of conditions. Specify Your Own Reaction ConditionsChoose either Taq with Mg-free 10X Reaction Buffer and separate 25mM MgCl2 or Taq with 10X Reaction Buffer containing 15mM MgCl2. Rely on a Performance-Tested EnzymeOur PCR systems, enzymes and reagents are proven in PCR to ensure reliable, high-performance results. If you are not completely satisfied with any of our PCR product, we will send a replacement or refund your account. Unit Defenition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10nmol of dNTP into acid-insoluble material in 30 minutes at 74 degree C. The reaction conditions are: 50mM Tris-HCl (pH 9.0 at 25 degree C), 50mM NaCl, 5mM MgCl2, 200um each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), 10?g activated calf thymus DNA and 0.1mg/ml BSA in a final volume of 50ul. 10X Reaction Buffer with MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C), 1% Triton X-100 and 15mM MgCl2. Buffer is optimized for use with 0.2mM of each dNTP. 10X Reaction Buffer without MgCl2500mM KCl, 100mM Tris-HCl (pH 9.0 at 25C) and 1% Triton X-100. Storage Buffer: Compatibility with Reaction Buffers: Taq DNA Polymerase in Storage Buffer. Use of other reaction buffers that do not contain Triton X-100 (final concentration of 0.1%) will result in inactivation of the enzyme. 50mM Tris-HCl (pH 8.0), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 50% glycerol and 1% Triton X-100.
other info2 :
QC Test: PCR, activity, SDS-PAGE/purity, endonuclease/nickase.
products categories :
ENZYMES; Enzymes; DNA Polymerase
products description :
Description: Taq DNA Polymerase(a) is a thermostable enzyme of approximately 94 kDa isolated from Thermus aquaticus. This unmodified enzyme replicates DNA at 74 degree C and exhibits a half-life of 40 minutes at 95 degree C. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5 ´~3 ´ direction in the presence of magnesium and also possesses a 5 ´~3 ´ exonuclease activity. Taq DNA Polymerase is recommended for use in PCR but is not recommended for use in DNA sequencing reactions.
ncbi gi num :
118828
ncbi acc num :
P19821.1
uniprot acc num :
P19821
ncbi mol weight :
93,910 Da
size1 :
1000 Units
price1 :
140 USD
size2 :
3000 Units
price2 :
205
size3 :
10000 Units
price3 :
415
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
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