protein

Recombinant Human DNA Topoisomerase-I

MBS142447

0.005 mg

140 USD

Recombinant Protein

Recombinant Human DNA Topoisomerase-I

DNA Topoisomerase-I

TOP1 Human; DNA Topoisomerase-I Human Recombinant; DNA topoisomerase 1; EC 5.99.1.2; DNA topoisomerase I; TOP1; Scl-70

DNA topoisomerase 1; DNA topoisomerase 1; DNA topoisomerase 1; type I DNA topoisomerase; topoisomerase (DNA) I; DNA topoisomerase I

TOP1

TOP1; TOP1; TOPI

TOP1_HUMAN

Sf9 Insect Cells

765

Greater than 95% as determined by SDS-PAGE.

TOP1 is supplied at a concentration of 0.92mg/1ml in 16mM HEPES buffer pH 7.5, 400mM NaCl and 20% glycerol. Physical Appearance: Sterile Filtered clear solution.

0.92mg/1ml

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

ENZYMES; Enzymes; Isomerase

Description: DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton (excluding glycosilation) 102 kDa total mass. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Introduction: DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antibodies are a specific marker in Scleroderma patients (specificity 98-100%) and are associated with the presence of diffuse skin involvement and pulmonary fibrosis.In human tissues the DNA topoisomerase I is initially synthesized as a protein with 100 kDa molecular weight. Most of this precursor is then proteolytically processed to a species with 70 kDa molecular weight from which the Scl-70 antigen has derived its name.

11225260

NP_003277.1

NM_003286.2

P11387

90,726 Da

Caspase Cascade In Apoptosis Pathway (137974); Integrated Pancreatic Cancer Pathway (711360)

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]

TOP1: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3 -phosphotyrosyl)-enzyme intermediate and the expulsion of a 5 -OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5 -OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. A chromosomal aberration involving TOP1 is found in a form of therapy-related myelodysplastic syndrome. Translocation t(11;20)(p15;q11) with NUP98. Belongs to the type IB topoisomerase family. Protein type: EC 5.99.1.2; Nucleolus; Oncoprotein; Isomerase. Chromosomal Location of Human Ortholog: 20q12-q13.1. Cellular Component: nucleoplasm; nuclear chromosome; nucleolus; perikaryon; nucleus. Molecular Function: DNA topoisomerase (ATP-hydrolyzing) activity; protein binding; DNA binding; chromatin binding; DNA topoisomerase type I activity. Biological Process: response to drug; chromatin remodeling; circadian rhythm; DNA topological change; viral reproduction; programmed cell death; circadian regulation of gene expression; DNA replication; phosphorylation; embryonic cleavage; chromosome segregation

0.005 mg

140 USD

0.02 mg

205

1 mg

3775