Recombinant Human Alanyl t-RNA Synthetase | MyBioSource MBS142446 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Alanyl t-RNA Synthetase

catalog :

MBS142446

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142446

products type :

Recombinant Protein

products full name :

Recombinant Human Alanyl t-RNA Synthetase

products short name :

Alanyl t-RNA Synthetase

products name syn :

PL 12 Human; Alanyl-tRNA Synthetase Human Recombinant; Alanyl-tRNA synthetase cytoplasmic; EC 6.1.1.7; Alanine-tRNA ligase; AlaRS; Renal carcinoma antigen NY-REN-42; PL-12; AARS

other names :

alanine--tRNA ligase, cytoplasmic; Alanine--tRNA ligase, cytoplasmic; alanine--tRNA ligase, cytoplasmic; AARS; alaRS; alanine tRNA ligase 1, cytoplasmic; alanyl-tRNA synthetase, cytoplasmic; renal carcinoma antigen NY-REN-42; alanyl-tRNA synthetase; Alanyl-tRNA synthetase

products gene name :

PL 12

other gene names :

AARS; AARS; CMT2N

uniprot entry name :

SYAC_HUMAN

host :

Sf9 Insect Cells

sequence length :

968

purity :

Greater than 90% as determined by SDS-PAGE.

form :

PL-12 is supplied in16mM HEPES buffer pH-8, 250mM sodium chloride, and 20% glycerol. Sterile Filtered clear solution.

concentration :

0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

other info2 :

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

products categories :

ENZYMES; Enzymes; Synthetase

products description :

Description: Alanyl-tRNA synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 107,598 Dalton. PL-12 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Introduction: Alanyl-tRNA synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 108 kDa protein is an autoantigen recognized by PL-12 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that epitope spreading occurs in the autoimmune PL-12 response such that even antibodies to an isolated alanyl-tRNA molecule can develop.

ncbi gi num :

109148542

ncbi acc num :

NP_001596.2

ncbi gb acc num :

NM_001605.2

uniprot acc num :

P49588

ncbi mol weight :

109,317 Da

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); TRNA Aminoacylation Pathway (105980)

ncbi summary :

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure. [provided by RefSeq, Jul 2008]

uniprot summary :

AARS: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain. Defects in AARS are the cause of Charcot-Marie-Tooth disease type 2N (CMT2N). It is an axonal form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathies(designated CMT1 when they are dominantly inherited) and primary peripheral axonal neuropathies (CMT2). Neuropathies of the CMT2 group are characterized by signs of axonal regeneration in the absence of obvious myelin alterations, normal or slightly reduced nerve conduction velocities, and progressive distal muscle weakness and atrophy. Nerve conduction velocities are normal or slightly reduced. Belongs to the class-II aminoacyl-tRNA synthetase family. Protein type: RNA-binding; Ligase; EC 6.1.1.7; Translation. Chromosomal Location of Human Ortholog: 16q22. Cellular Component: membrane; cytoplasm; cytosol. Molecular Function: amino acid binding; zinc ion binding; ATP binding; alanine-tRNA ligase activity; tRNA binding. Biological Process: protein folding; unfolded protein response; tRNA processing; regulation of translational fidelity; cerebellar Purkinje cell layer development; tRNA modification; response to amino acid stimulus; tRNA aminoacylation for protein translation; hair follicle development; gene expression; negative regulation of neuron apoptosis; neuromuscular process controlling balance; alanyl-tRNA aminoacylation. Disease: Epileptic Encephalopathy, Early Infantile, 29; Charcot-marie-tooth Disease, Axonal, Type 2n

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3735

more info or order :

MyBioSource product webpage