Recombinant Human Tissue Transglutaminase, Sf9 | MyBioSource MBS142444 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Tissue Transglutaminase, Sf9

catalog :

MBS142444

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142444

products type :

Recombinant Protein

products full name :

Recombinant Human Tissue Transglutaminase, Sf9

products short name :

Tissue Transglutaminase

products name syn :

TGM2 Human, Sf9; Tissue Transglutaminase Human Recombinant, Sf9; Protein-glutamine gamma-glutamyltransferase 2; EC 2.3.2.13; Tissue transglutaminase; TGase C; TGC; TG(C); Transglutaminase-2; TGase-H; TG2; TGM2

other names :

protein-glutamine gamma-glutamyltransferase 2 isoform a; Protein-glutamine gamma-glutamyltransferase 2; protein-glutamine gamma-glutamyltransferase 2; C polypeptide, protein-glutamine-gamma-glutamyltransferase; TG(C); TGase C; TGase H; TGase-2; TGase-H; epididymis secretory protein Li 45; tissue transglutaminase; transglutaminase C; transglutaminase H; transglutaminase-2; transglutaminase 2; Tissue transglutaminase; Transglutaminase C; TG(C); TGC; TGase C; Transglutaminase H; TGase H; Transglutaminase-2; TGase-2

products gene name :

TGM2 Sf9

other gene names :

TGM2; TGM2; TG2; TGC; GNAH; HEL-S-45; G-ALPHA-h; TG(C); TGC; TGase C; TGase H; TGase-2

uniprot entry name :

TGM2_HUMAN

host :

Sf9 Insect Cells

sequence length :

687

purity :

Greater than 95% as determined by SDS-PAGE.

form :

TGM2 is supplied in 16mM HEPES buffer pH-8.0, 320mM NaCl, and 20% glycerol.

concentration :

Coating Concentration: 0.6-1.4 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

products categories :

ENZYMES; Enzymes

products description :

Description: Tissue Transglutaminase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 78,018 Dalton. tTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating. Introduction: Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig.Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme's secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins.

ncbi gi num :

39777597

ncbi acc num :

NP_004604.2

ncbi gb acc num :

NM_004613.2

uniprot acc num :

P21980

ncbi mol weight :

38,671 Da

ncbi pathways :

Huntington's Disease Pathway (83100); Huntington's Disease Pathway (512); Thromboxane A2 Receptor Signaling Pathway (138060)

ncbi summary :

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Finally, the encoded protein is the autoantigen implicated in celiac disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TGM2: an enzyme of the transglutaminase family that catalyzes the crosslinking of proteins and the conjugation of polyamines to proteins. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. Is the autoantigen in coeliac disease and plays a role in apoptosis, cellular differentiation and matrix stabilisation. Three alternatively spliced isoforms have been described. Protein type: Transferase; EC 2.3.2.13. Chromosomal Location of Human Ortholog: 20q12. Cellular Component: focal adhesion; mitochondrion; plasma membrane; cytosol. Molecular Function: protein domain specific binding; protein binding; GTP binding; protein-glutamine gamma-glutamyltransferase activity; metal ion binding. Biological Process: elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of apoptosis; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of smooth muscle cell proliferation; blood vessel remodeling; protein homooligomerization; apoptotic cell clearance; positive regulation of inflammatory response; elevation of mitochondrial calcium ion concentration

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

4345

more info or order :

MyBioSource product webpage