Recombinant Human Secreted Phospholipase A2-IIA | MyBioSource MBS142431 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Secreted Phospholipase A2-IIA

catalog :

MBS142431

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142431

products type :

Recombinant Protein

products full name :

Recombinant Human Secreted Phospholipase A2-IIA

products short name :

Secreted Phospholipase A2-IIA

products name syn :

PLA2G2A Human; Secreted Phospholipase A2-IIA Human Recombinant; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; Phospholipase A2 membrane associated; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase; Group IIA phospholipase A2; GIIC sPLA2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; sPLA2-IIA; PLA2G2A

other names :

phospholipase A2, membrane associated; Phospholipase A2, membrane associated; phospholipase A2, membrane associated; GIIC sPLA2; NPS-PLA2; group IIA phospholipase A2; non-pancreatic secretory phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; phospholipase A2, group IIA (platelets, synovial fluid); GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A

products gene name :

PLA2G2A

other gene names :

PLA2G2A; PLA2G2A; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; PLA2B; PLA2L; RASF-A; NPS-PLA2

uniprot entry name :

PA2GA_HUMAN

host :

E Coli

sequence length :

144

purity :

Greater than 95% as determined by SDS-PAGE.

form :

Lyophilized from 0.5 mg/ml in 0.05M Acetate buffer pH-4. Sterile Filtered lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

other info2 :

Solubility: Add 0.2 ml of 0.1M Acetate buffer pH-4 and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 ug/ml. In higher concentrations the solubility of this antigen is limited.

products categories :

ENZYMES; Enzymes; Secreted Phospholipase A2

products description :

Description: Secreted Phospholipase A2-IIA Human Recombinant is manufactured with N-terminal fusion of HisTag. PLA2G2A His-Tagged Fusion Protein is 15.8 kDa containing 124 amino acid residues of the human secreted phospholipase A2-IIA and 16 additional amino acid residues HisTag. Introduction: Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.sPLA2-IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of sPLA2-IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.

ncbi gi num :

4505849

ncbi acc num :

NP_000291.1

ncbi gb acc num :

NM_000300.3

uniprot acc num :

P14555

ncbi mol weight :

16,083 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

ncbi summary :

The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009]

uniprot summary :

PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family. Protein type: Lipid Metabolism - arachidonic acid; Mitochondrial; Lipid Metabolism - ether lipid; Secreted, signal peptide; Phospholipase; Vesicle; Secreted; Lipid Metabolism - alpha-linolenic acid; EC 3.1.1.4; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - linoleic acid; Endoplasmic reticulum. Chromosomal Location of Human Ortholog: 1p35. Cellular Component: extracellular space; endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; extracellular region; secretory granule. Molecular Function: phospholipase A2 activity; calcium-dependent phospholipase A2 activity; phospholipid binding; calcium ion binding. Biological Process: defense response to Gram-positive bacterium; somatic stem cell maintenance; phosphatidic acid metabolic process; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process; negative regulation of epithelial cell proliferation; positive regulation of inflammatory response. Disease: Colorectal Cancer

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4140

more info or order :

MyBioSource product webpage