catalog number :
MBS142430
products type :
Recombinant Protein
products full name :
Recombinant Yeast Isocitrate Dehydrogenase-1
products short name :
Isocitrate Dehydrogenase-1
products name syn :
IDH1; Isocitrate Dehydrogenase-1 Yeast Recombinant; Isocitrate dehydrogenase [NADP] cytoplasmic; EC 1.1.1.42; Cytosolic NADP-isocitrate dehydrogenase; Oxalosuccinate decarboxylase; IDH; NADP(+)-specific ICDH; IDP; PICD
other names :
isocitrate dehydrogenase; Peroxisomal isocitrate dehydrogenase [NADP]; isocitrate dehydrogenase
products gene name :
IDH1
other gene names :
ICDH; ICDH; F20D21.16; F20D21_16; ICDH; isocitrate dehydrogenase
uniprot entry name :
ICDHX_ARATH
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
One ml of solution contains 0.075 mol/l KPO4, 50% Glycerol, pH 7.1. Sterile Filtered clear solution.
other info1 :
Unit Defenition: One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25 degree C, pH 7.5).
other info2 :
Biological Activity: The specific activity was found to be 115 U/mg.
products categories :
ENZYMES; Enzymes; Dehydrogenase
products description :
Description: Recombinant Saccharomyces Cerevisiae ICDH (NADP) derived from yeast host cells by using over-expression system, is full length same as designated ICD1 from Saccharomyces Cerevisiae. The N-terminal amino acid Phenylalanine residue next to Met is substituted with Alanine for overexpression. The ICDH is purified by proprietary chromatographic techniques. Introduction: Isocitrate Dehydrogenase is an enzyme of the oxidoreductase class that catalyzes the conversion of isocitrate and NAD+ to yield 2-ketoglutarate, carbon dioxide, and NADH. It occurs in cell mitochondria. The enzyme requires Mg2+, Mn2+; it is activated by ADP, citrate, and Ca2+, and inhibited by NADH, NADPH, and ATP. The reaction is the key rate-limiting step of the citric acid (tricarboxylic) cycle.
ncbi acc num :
NP_175836.1
ncbi gb acc num :
NM_104312.2
ncbi mol weight :
47,234 Da
ncbi pathways :
2-Oxocarboxylic Acid Metabolism Pathway (714629); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Abnormal Conversion Of 2-oxoglutarate To 2-hydroxyglutarate Pathway (1118525); Biosynthesis Of Amino Acids Pathway (790118); Biosynthesis Of Amino Acids Pathway (795174); Biosynthesis Of Secondary Metabolites Pathway (148695); Carbon Metabolism Pathway (815031); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (3985); Citrate Cycle (TCA Cycle) Pathway (288)
ncbi summary :
NADP-specific isocitrate dehydrogenase (ICDH)