Recombinant Human Thyroid Peroxidase | MyBioSource MBS142426 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Thyroid Peroxidase

catalog :

MBS142426

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142426

products type :

Recombinant Protein

products full name :

Recombinant Human Thyroid Peroxidase

products short name :

Thyroid Peroxidase

products name syn :

TPO Human; Thyroid Peroxidase Human Recombinant; Thyroid peroxidase; EC 1.11.1.8; TPO; MSA; TPX

other names :

thyroid peroxidase isoform a; Thyroid peroxidase; thyroid peroxidase; thyroid microsomal antigen; thyroperoxidase; thyroid peroxidase

products gene name :

TPO

other gene names :

TPO; TPO; MSA; TPX; TDH2A; TPO

uniprot entry name :

PERT_HUMAN

host :

Sf9 Insect Cells

sequence length :

933

purity :

Greater than 95% as determined by SDS-PAGE.

form :

TPO is supplied in 16mM HEPES pH-7.6, 160mM NaCl, 0.08mM Kl and 20% glycerol.

concentration :

0.15-0.375 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

other info2 :

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Auto-antibodies to TPO recognize conformation-dependent epitopes.3. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).

products categories :

ENZYMES; Enzymes; Oxidase

products description :

Description: Thyroid Peroxidase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain containing 834 amino acids and having a molecular mass of 92,872 Dalton (excluding glycosylation), 101 kDa total mass.The TPO is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Introduction: Thyroid Peroxidase (TPO) represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. Its identity with the formerly so-called `microsomal antigen` has been shown several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx. 100 kDa molecular weight. The hemoprotein TPO plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin (TG) to form precursors of the thyroid hormones T4 and T3.

ncbi gi num :

28558982

ncbi acc num :

NP_000538.3

ncbi gb acc num :

NM_000547.5

uniprot acc num :

P07202

ncbi mol weight :

92,063 Da

ncbi pathways :

Amine-derived Hormones Pathway (160983); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Thyroid Hormone Biosynthesis, Tyrosine = Triiodothyronine/thyroxine Pathway (413358); Thyroid Hormone Biosynthesis, Tyrosine = Triiodothyronine/thyroxine Pathway (468236); Thyroid Hormone Synthesis Pathway (835410); Thyroid Hormone Synthesis Pathway (839541)

ncbi summary :

This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011]

uniprot summary :

TPO: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). An alternative splicing in the thyroperoxidase mRNA can cause Graves disease. Defects in TPO are the cause of thyroid dyshormonogenesis 2A (TDH2A). A disorder due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete. Belongs to the peroxidase family. XPO subfamily. 8 isoforms of the human protein are produced by alternative splicing. Protein type: EC 1.11.1.8; Oxidoreductase; Amino Acid Metabolism - tyrosine; Mitochondrial; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: extracellular space; cell surface; mitochondrion; integral to plasma membrane; plasma membrane. Molecular Function: peroxidase activity; heme binding; iodide peroxidase activity; calcium ion binding. Biological Process: hydrogen peroxide catabolic process; embryonic hemopoiesis; thyroid hormone generation; response to oxidative stress; hormone biosynthetic process. Disease: Thyroid Dyshormonogenesis 2a

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

4820

more info or order :

MyBioSource product webpage