protein

Recombinant Human Glycogen Phosphorylase

MBS142423

0.02 mg

205 USD

Recombinant Protein

Recombinant Human Glycogen Phosphorylase

Glycogen Phosphorylase

GPBB Human; Glycogen Phosphorylase Human Recombinant; Glycogen phosphorylase brain form; EC 2.4.1.1; GPBB; MGC9213; PYGB

glycogen phosphorylase, brain form; Glycogen phosphorylase, brain form; glycogen phosphorylase, brain form; glycogen phosphorylase B; phosphorylase, glycogen; brain

GPBB

PYGB; PYGB; GPBB

PYGB_HUMAN

E Coli

843

Greater than 85.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Each mg of protein contains 50% glycerol. Sterile Filtered colourless liquid formualtion.

GPBB although stable at 10 degree C for 7 days, should be stored below -18 degree C. Please prevent freeze-thaw cycles.

Immunoassay, Western Blot (WB)

ENZYMES; Enzymes; Phosphorylase

Description: Glycogen Phosphorylase Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain. The Human GPBB mature chain: 2 - 843 aa; that is a total of 842 aa having a molecular mass of 96695.96 Dalton. The theoretical pI is 6.40.The GPBB is purified by proprietary chromatographic techniques. Introduction: Glycogen phosphorylase is one of the phosphorylaseenzymes(EC2.4.1.1). It breaks up glycogeninto glucosesubunits. Glycogenis left with one less glucosemolecule, and the free glucosemolecule is in the form of glucose-1-phosphate. In order to be used for metabolism, it must be converted to glucose-6-phosphateby the enzyme phosphoglucomutase.Glycogen phosphorylase can only act on linearchainsof glycogen(a 1-4 glycosidic linkage). Its work will immediately come to a halt four residues away from a 1-6 branch(which are exceedingly common in glycogen). In these situations, a debranching enzymeis necessary, which will straighten out the chain in that area. Additionally, an alpha 1-6 glucosidaseenzymeis required to break the remaining 1-6 residue that remains in the new linear chain. After all this is done, glycogen phosphorylase can continue.An insulinstimulated enzyme known as phosphoprotein phosphatase(PP-1) inactivates glycogen phosphorylase to prevent glycogen break up.GPBB - a sensitive marker for the AMI diagnosis within 4 hours after the onset of chest pain. It has also been shown that GPBB is increased in a considerable proportion of AMI patients within 2-3 hours from chest pain onset. GPBB is increased early in patients with unstable angina. GPBB can also be a sensitive marker for the detection of peri-operative myocardial ischaemia and infarction in patients undergoing coronary artery bypass grafting.

21361370

NP_002853.2

NM_002862.3

P11216

96,696 Da

Disease Pathway 530764!!Glucose Metabolism Pathway 106199!!Glycogen Metabolism Pathway 198856!!Glycogen Breakdown (glycogenolysis) Pathway 106212!!Glycogen Storage Diseases Pathway 1127581!!Insulin Signaling Pathway 83090!!Insulin Signaling Pathway 501!!Metabolic Pathways 132956!!Metabolism Pathway 477135!!Metabolism Of Carbohydrates Pathway 106196

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq, Jul 2008]

0.02 mg

205 USD