protein

Recombinant Pyrococcus Fruriosus Deoxyuridine Triphosphatase

MBS142422

500 IU

205 USD

Recombinant Protein

Recombinant Pyrococcus Fruriosus Deoxyuridine Triphosphatase

Pyrococcus Fruriosus Deoxyuridine Triphosphatase

DUT Pyrococcus Fruriosus; Thermostable dUTPase Pyrococcus Fruriosus Recombinant; Thermostable dUTPase; dUTPase

Deoxyuridine 5'-triphosphate nucleotidohydrolase; Deoxyuridine 5'-triphosphate nucleotidohydrolase; dUTP pyrophosphatase

DUT

U45; BHLF3; dUTPase

DUT_HHV6U

E Coli

376

Greater than 97.0% as determined by SDS-PAGE.

dUTPase is supplied in 20mM Tris-HCl (pH 8.2), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at a concentration of 10U/ ul of the enzyme. Sterile filtered liquid formulation.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Unit Defenition: One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade. Specific Activity: 10 ³u/ug.

Activity: (A) Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepes pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma.(B) Enhancing PCR amplification: 50ul of Pfu PCR reaction system with 1-3u of dUTPase ( 3.0kb) to amplify genomic DNA target up to 15-19 kb in length. (High concentrations of dUTPase will inhibit PCR reaction!)

ENZYMES; Enzymes; Phosphatase

Description: Thermostable dUTPase (pyrococcus fruriosus) maximizes the efficiency of high-fidelity PCR (using proofreading DNA polymerases). It removes contaminating dUTP present in PCR reactions and dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall and slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications such as cloning and subsequent recombinant protein technology, and gene expression analysis (semi-quantitative RT-PCR techniques and real-time PCR analysis), where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair. dUTPase hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing dUTP levels and providing the dUMP for dTTP biosynthesis.

1169439

Q06095.1

43,398 Da

500 IU

205 USD

1000 IU

265

2000 IU

360