Recombinant Human Glutamic-Pyruvate Transaminase | MyBioSource MBS142421 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Glutamic-Pyruvate Transaminase

catalog :

MBS142421

quantity :

1 Unit

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142421

products type :

Recombinant Protein

products full name :

Recombinant Human Glutamic-Pyruvate Transaminase

products short name :

Glutamic-Pyruvate Transaminase

products name syn :

GPT Human, Active; Glutamic-Pyruvate Transaminase Human Recombinant, Active; Alanine aminotransferase 1; ALT1; EC 2.6.1.2; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1; GPT; AAT1; GPT1; GPT Active

other names :

alanine aminotransferase 1; Alanine aminotransferase 1; alanine aminotransferase 1; GPT 1; glutamate pyruvate transaminase 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamic-alanine transaminase 1; glutamic-pyruvate transaminase (alanine aminotransferase); Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1

products gene name :

GPT

other gene names :

GPT; GPT; AAT1; ALT1; GPT1; AAT1; GPT1; ALT1; GPT 1

uniprot entry name :

ALAT1_HUMAN

host :

E Coli

sequence length :

496

purity :

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein (1,720U/ml) was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate. Sterile liquid formulation.

storage stability :

AAT1 although stable at 10 degree C for 5 days, should be stored below -18 degree C.Please prevent freeze-thaw cycles.

other info2 :

Biological Activity: The specific activity was found to be 1,020 U/mg.

products categories :

ENZYMES; Enzymes; Transaminase

products description :

Description: Alanine Aminotransferase Human Recombinant produced in E Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT.The ALT is purified by proprietary chromatographic techniques. Introduction: Alanine transaminase or ALT is a transaminaseenzyme.ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamatealanine+ a-ketoglutarate= pyruvate+ glutamateIt is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L).

ncbi gi num :

4885351

ncbi acc num :

NP_005300.1

ncbi gb acc num :

NM_005309.2

uniprot acc num :

P24298

ncbi pathways :

2-Oxocarboxylic Acid Metabolism Pathway (714485); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198783); Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (106173); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567)

ncbi summary :

This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]

uniprot summary :

GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily. Protein type: EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: cytosol. Molecular Function: alanine transaminase activity; pyridoxal phosphate binding. Biological Process: amino acid biosynthetic process; L-alanine catabolic process

size1 :

1 Unit

price1 :

140 USD

size2 :

3 Units

price2 :

205

size3 :

50 Units

price3 :

1790

more info or order :

MyBioSource product webpage