catalog number :
MBS142420
products type :
Recombinant Protein
products full name :
Recombinant Lactate Dehydrogenase B
products short name :
Lactate Dehydrogenase B
products name syn :
LDHB; Lactate Dehydrogenase B Recombinant; Lactate Dehydrogenase; LDH
other names :
ldh, partial; L-lactate dehydrogenase 1
products gene name :
LDHB
other gene names :
ldh1; ldh; L-LDH 1
uniprot entry name :
LDH1_LISMO
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein (1 mg/ml) was lyophilized with 0.1mg potassium phosphate. Sterile lyophilized powder.
storage stability :
Lyophilized Lactate Dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution LDH should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
other info1 :
Unit Defenition: One unit is defined as 1umol of NAD+ production per minute under the assay conditions (25 degree C, pH 7.0). Both transaminase activities include a-hydroxyglutarate dehydrogenase activity.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized LDH in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The specific activity was found to be 258 U/mg protein.
products categories :
ENZYMES; Enzymes; Dehydrogenase
products description :
Description: The DNA encoding chicken LDH-B is cloned from cDNA library of chicken heart. Introduction: Lactate dehydrogenase (LDH) is an enzyme(EC1.1.1.27) present in a wide variety of organisms, including plants and animals.A tetrameric enzyme that catalyses the interconversion of pyruvateand lactate with concomitant interconversion of NADH and NAD+. At high concentrations of pyruvate, the enzyme exhibits feedback inhibition and the rate of conversion of pyruvate to lactate is decreased. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist.
ncbi acc num :
AAA25274.1
ncbi mol weight :
34,192 Da