product summary
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company name :
MyBioSource
product type :
protein
product name :
Recombinant Urease
catalog :
MBS142418
quantity :
1 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142418
products type :
Recombinant Protein
products full name :
Recombinant Urease
products short name :
Urease
products name syn :
Urease; Urease Recombinant; Urease
other names :
urease; urease
other gene names :
URE; F12B7.10; F12B7_10; URE; urease
host :
E Coli
sequence length :
838
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Each mg of protein contains 420 ug Potassium Phosphate and 30 ug EDTA Na2. Sterile Lyophilized Powder.
storage stability :
Urease although stable at 4 degree C for 3 weeks, should be stored desiccated below -18 degree C. Please prevent freeze-thaw cycles.
other info1 :
Unit Defenition: One Unit oxidizes one micromole of NADH per minute at 25 degree C, at pH 7.6.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Urease in sterile 18M Omega -cm H2O. Biological Activity: The activity was found to be 141U/mg powder.
products categories :
ENZYMES; Enzymes
products description :
Description: Urease (EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O = CO2 + 2NH3. In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and several higher plants. Characteristics: Active site metal: nickel(II); Molecular weight: 480 kDa or 545 kDa for Jack Bean Urease (calculated mass from the amino acid sequence); Optimum pH: 7.4; Optimum Temperature: 60 degrees Celsius; Enzymatic specificity: urea and hydroxyurea; Inhibitors: heavy metals. The multi-subunit enzyme usually has a 3:3 (alpha:beta) stoichiometry with a 2-fold symmetric structure (note that the image above gives the structure of the asymmetric unit, one third of the true biological assembly). An exceptional urease is found in Helicobacter pylori, which combines four of the regular six subunit enzymes in an overall tetrahedral assembly of 24 subunits (a12b12). This supra-molecular assembly is thought to confer additional stability for the enzyme in this organism, which functions to produce ammonia in order to neutralise gastric acid. The presence of urease is used in the diagnosis of Helicobacter species.
ncbi gi num :
15220459
ncbi acc num :
NP_176922.1
ncbi gb acc num :
NM_105422.3
ncbi pathways :
Arginine And Proline Metabolism Pathway (4017); Arginine And Proline Metabolism Pathway (323); Metabolic Pathways (132014); Purine Metabolism Pathway (4004); Purine Metabolism Pathway (307); Superpathway Of Allantoin Degradation In Plants (908237); Urea Degradation II Pathway (546154)
ncbi summary :
Encodes a nickel-containing urea hydrolase involved in nitrogen recycling. It requires three urease accessory proteins for its activation.
size1 :
1 mg
price1 :
140 USD
size2 :
5 mg
price2 :
205
size3 :
50 mg
price3 :
1015
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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