catalog number :
MBS142417
products type :
Recombinant Protein
products full name :
Recombinant Disulfide Oxidoreductase
products short name :
Disulfide Oxidoreductase
products name syn :
DsbA; Disulfide Oxidoreductase Recombinant; DsbA; Thiol:disulfide interchange protein dsbA
other names :
periplasmic protein disulfide isomerase I; Thiol:disulfide interchange protein DsbA; periplasmic protein disulfide isomerase I
products gene name :
DsbA
other gene names :
dsbA; dsbA; dsf; ECK3852; iarA; JW3832; ppfA; dsf; ppfA
uniprot entry name :
DSBA_ECOLI
sequence :
The sequence of the first five N-terminal amino acidswas determined and was found to be Met-Ly-Lys-Ala-Trp.
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized after from a sterile solution containing 50mM sodium phosphate buffer and 100mM sodium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized DsbA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution DsbA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized DsbA in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
products categories :
ENZYMES; Enzymes; Reductase
products description :
Description: Disulfide Oxidoreductase produced in E Coli is a periplasmic protein isolated from E Coli, containing 208 amino acids having a molecular mass of 23,149 Dalton. The DsbA is purified by proprietary chromatographic techniques. Introduction: DsbA appears to be necessary for correct formulation of disulfide bonds in exported proteins in vivo. DsbA is useful as a standard in immunoblotting. This protein catalyses the reduction and exchange of disulfide bonds and the oxidation of free sulfhydryl groups in vitro. It is the strongest oxidant of the thioredoxin superfamily. This thio/disulfide oxidoreductase is required for efficient disulfide bond formation in the periplasm of E. coli.
ncbi acc num :
NP_418297.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
23,105 Da
ncbi summary :
DsbA has a role in cytochrome C synthesis. [More information is available at EcoGene: EG11297].