protein

Recombinant Human Ubiquitin-Conjugating Enzyme E2I, His tag

MBS142415

0.01 mg

140 USD

Recombinant Protein

Recombinant Human Ubiquitin-Conjugating Enzyme E2I, His tag

Ubiquitin-Conjugating Enzyme E2I

UBE2I Human His; Ubiquitin-Conjugating Enzyme E2I Human Recombinant, His Tag; SUMO-conjugating enzyme UBC9; EC 6.3.2.-; SUMO-protein ligase; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; Ubiquitin carrier protein I; Ubiquitin carrier protein 9; p18; UBC9; C358B7.1; UBE2I His

SUMO-conjugating enzyme UBC9; SUMO-conjugating enzyme UBC9; SUMO-conjugating enzyme UBC9; SUMO-1-protein ligase; SUMO-protein ligase; ubiquitin carrier protein 9; ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; ubiquitin-protein ligase I; ubiquitin-conjugating enzyme E2I; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18

UBE2I

UBE2I; UBE2I; P18; UBC9; C358B7.1; UBC9; UBCE9

UBC9_HUMAN

E Coli

158

MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVA
VPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFK
DDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWR
PAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVE
YEKRVRAQAKKFAPS.

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a 0.2um filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5. Sterile Filtered white lyophilized powder.

Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution UBE2I should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

ENZYMES; Enzymes; Ubiquitin Conjugating Enzyme

Description: Ubiquitin-Conjugating Enzyme E2I Human Recombinant produced in E Coli is a 19.5 kDa protein containing 171 amino acids.The UBE2I protein contains 6xHis tag and is purified by proprietary chromatographic techniques. Introduction: Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1, I?B?, and PML without the requirement of an E3 ligase.

4507785

NP_003336.1

NM_003345.4

P63279

18,007 Da

Androgen Receptor Signaling Pathway 198806!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 530733!!Coregulation Of Androgen Receptor Activity Pathway 138085!!Meiosis Pathway 477133!!Meiotic Synapsis Pathway 477134!!Metabolism Of Proteins Pathway 106230!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!NF-kappa B Signaling Pathway 634527

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

0.01 mg

140 USD