catalog number :
MBS142414
products type :
Recombinant Protein
products full name :
Recombinant Malate Dehydrogenase 1
products short name :
Malate Dehydrogenase 1
products name syn :
MDH1 Chicken; Malate Dehydrogenase Chicken Recombinant; Malate dehydrogenase cytoplasmic; EC 1.1.1.37; Cytosolic malate dehydrogenase; MDHA; MOR2; MDH-s; MGC:1375; MDH1
other names :
malate dehydrogenase, cytoplasmic; Malate dehydrogenase, cytoplasmic; malate dehydrogenase, cytoplasmic; cytosolic malate dehydrogenase; malate dehydrogenase 1, NAD (soluble); Cytosolic malate dehydrogenase
products gene name :
MDH1
other gene names :
MDH1; MDH1
uniprot entry name :
MDHC_CHICK
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Each mg of protein contains 0.59mg NaPO4. Sterile lyophilized powder.
storage stability :
Lyophilized Malate dehydrogenase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution MDH1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.
other info1 :
Unit Defenition: One unit is defined as 1 umol of NAD+ production per minute under the assay conditions (25 degree C, pH 7.5). Specific Activity: Specific Activity Greater than 710U/mg protein.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Malate dehydrogenase in sterile 18M Omega -cm H2O.
products categories :
ENZYMES; Enzymes; Dehydrogenase
products description :
Description: The DNA encoding Malate (Malic) Dehydrogenase is cloned from cDNA library of chicken heart.The MDH1 is purified by proprietary chromatographic techniques. Introduction: Malate dehydrogenase (EC1.1.1.37) is an enzyme in the citric acid cycle that catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is not to be confused with malic enzyme, which catalyzes the conversion of pyruvate using NADPH.Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Pyruvate in the mitochondria is acted upon by pyruvate carboxylase to form oxaloacetate, a citric acid cycle intermediate. In order to get the oxaloacetate out of the mitochondria, malate dehydrogenase reduces it to malate, and it then traverses the inner mitochondrial membrane. Once in the cytosol, the malate is oxidized back to oxaloacetate by cytosolic malate dehydrogenase. Finally, phosphoenol-pyruvate carboxy kinase (PEPCK) converts oxaloacetate to phosphoenol pyruvate.
ncbi acc num :
NP_001006395.1
ncbi gb acc num :
NM_001006395.1
ncbi mol weight :
36,543 Da
ncbi pathways :
Carbon Metabolism Pathway (815546); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (83839); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855806); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (583156); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204); Cysteine And Methionine Metabolism Pathway (104498); Cysteine And Methionine Metabolism Pathway (103421)