Recombinant Human Matrix Metalloproteinase-7 | MyBioSource MBS142412 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Matrix Metalloproteinase-7

catalog :

MBS142412

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142412

products type :

Recombinant Protein

products full name :

Recombinant Human Matrix Metalloproteinase-7

products short name :

Matrix Metalloproteinase-7

products name syn :

MMP 7 Human; Matrix Metalloproteinase-7 Human Recombinant; Matrilysin; EC 3.4.24.23; Pump-1 protease; Uterine metalloproteinase; Matrix metalloproteinase-7; MMP-7; Matrin; MPSL1; PUMP-1; MMP7

other names :

matrilysin preproprotein; Matrilysin; matrilysin; matrin; matrix metallopeptidase 7 (matrilysin, uterine); matrix metalloproteinase 7 (matrilysin, uterine); matrix metalloproteinase-7; pump-1 protease; uterine matrilysin; uterine metalloproteinase; matrix metallopeptidase 7; Matrin; Matrix metalloproteinase-7; MMP-7; Pump-1 protease; Uterine metalloproteinase

products gene name :

MMP 7

other gene names :

MMP7; MMP7; MMP-7; MPSL1; PUMP-1; MPSL1; PUMP1; MMP-7

uniprot entry name :

MMP7_HUMAN

host :

E Coli

sequence length :

267

purity :

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein contains the following additives 10mM HEPES (pH 7.4), 5mM CaCl2 and 150mM NaCl. Sterile clear liquid solution.

storage stability :

Matrilysin although stable at 4 degree C for 3 weeks, should be stored desiccated below -18 degree C. Please prevent freeze-thaw cycles.

other info1 :

Recommended Use: Western Blotting (1:1K-5K for neat serum and 1-10 ug/ml for affinity pure antibody using ECL technique). ELISA: Control peptide can be used to coat ELISA plates at 1 ug/ml and detected with antibodies (1:10-50K for neat serum and 0.5-1 ug/ml for affinity pure). Histochemistry & Immunofluorescence: Not tested. Unit Defenition: One unit is defined as the digestion of 1 ug Azocoll/min at 37 degree C.

other info2 :

Biological Activity: The specific activity was found to be 6,900 U/mg.

products categories :

ENZYMES; Enzymes; Matrix Metalloproteinase

products description :

Description: Matrix Metalloproteinase-7 Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing having a molecular mass of 19.13 kDa.The MMP-7 is purified by proprietary chromatographic techniques. Introduction: Matrix metalloproteinase-7 (MMP-7) also known as matrilysin and PUMP (EC 3.4.24.23) cleaves a number of substrates including collagen types IV and X, elastin, fibronectin, gelatin, laminin and proteoglycans. MMP-7 is closely related to the stromelysin family members but is encoded by a different gene. MMP-7 is the smallest of all the MMPs consisting of a pro-peptide domain and a catalytic domain. It lacks the hemopexin-like domain common to other members of the MMPs. MMP-7 is secreted as a 28 kDa proenzyme and can be activated in vitro by organomercurials and trypsin and in vivo by MMP-3 to a 18 kDa active MMP-7 enzyme. Once activated, MMP-7 can activate pro-MMP-1 and pro-MMP-9 but not pro-MMP-2. MMP-7 is widely expressed having been reported in elevated levels in cycling endometrium as well as in colorectal cancers and adenomas, hepatocellular carcinomas, rectal carcinomas, and approximately 50% of gliomas.

ncbi gi num :

4505219

ncbi acc num :

NP_002414.1

ncbi gb acc num :

NM_002423.3

uniprot acc num :

P09237

ncbi mol weight :

29,677 Da

ncbi pathways :

AGE/RAGE Pathway (698754); Activation Of Matrix Metalloproteinases Pathway (576264); Alpha6-Beta4 Integrin Signaling Pathway (198807); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900); Syndecan-1-mediated Signaling Events Pathway (138046)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal protein domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

uniprot summary :

MMP7: Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. Belongs to the peptidase M10A family. Protein type: Protease; Secreted, signal peptide; Secreted; EC 3.4.24.23. Chromosomal Location of Human Ortholog: 11q22.2. Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: zinc ion binding; metalloendopeptidase activity. Biological Process: response to drug; extracellular matrix disassembly; collagen catabolic process; defense response to Gram-positive bacterium; extracellular matrix organization and biogenesis; antibacterial peptide biosynthetic process; defense response to Gram-negative bacterium; proteolysis; antibacterial peptide secretion; regulation of cell proliferation

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage