Recombinant Human Histidyl-tRNA Synthetase | MyBioSource MBS142409 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Histidyl-tRNA Synthetase

catalog :

MBS142409

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142409

products type :

Recombinant Protein

products full name :

Recombinant Human Histidyl-tRNA Synthetase

products short name :

Histidyl-tRNA Synthetase

products name syn :

HARS Human; Histidyl-tRNA Synthetase Human Recombinant; Histidyl-tRNA synthetase; EC 6.1.1.21; Histidine-tRNA ligase; HisRS; HRS; FLJ20491; JO-1

other names :

histidine--tRNA ligase, cytoplasmic isoform 2; Histidine--tRNA ligase, cytoplasmic; histidine--tRNA ligase, cytoplasmic; HisRS; histidine translase; histidyl-tRNA synthetase; Histidyl-tRNA synthetase; HisRS

products gene name :

HARS

other gene names :

HARS; HARS; HRS; USH3B; HRS; HisRS

uniprot entry name :

SYHC_HUMAN

host :

E Coli

sequence length :

469

purity :

Greater than 95.0% as determined by both:(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein solution (0.718mg/ml) contains 500mM NaCl and 10mM Tris (pH 8.0) and 6M Urea. Sterile Filtered clear solution.

concentration :

0.718mg/ml

storage stability :

Histidyl-tRNA Synthetase although stable at 4 degree C for 3 weeks, should be stored below -18 degree C.Please prevent freeze-thaw cycles.

app notes :

Western Blot: Strongly reactive with human anti Histidyl-tRNA Synthetase antisera.

other info2 :

Protein Content: Protein quantitation was carried out by using 0.25 - 2.0 mg/ml Bradford assay vs. BSA.

products categories :

ENZYMES; Enzymes; Synthetase

products description :

Description: Histidyl-tRNA Synthetase Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 55 kDa. Introduction: Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.

ncbi gi num :

384475552

ncbi acc num :

NP_001244969.1

ncbi gb acc num :

NM_001258040.2

uniprot acc num :

P12081

ncbi mol weight :

54,847 Da

ncbi pathways :

Aminoacyl-tRNA Biosynthesis Pathway (83030); Aminoacyl-tRNA Biosynthesis Pathway (424); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (413439); Aminoacyl-tRNA Biosynthesis, Eukaryotes Pathway (468360); Cytosolic TRNA Aminoacylation Pathway (105981); Gene Expression Pathway (105937); TRNA Aminoacylation Pathway (105980)

ncbi summary :

Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

HARS: Defects in HARS are a cause of Usher syndrome type 3B (USH3B). USH3B is a syndrome characterized by progressive vision and hearing loss during early childhood. Some patients have the so-called Charles Bonnet syndrome, involving decreased visual acuity and vivid visual hallucinations. USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa with sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH3 is characterized by postlingual, progressive hearing loss, variable vestibular dysfunction, and onset of retinitis pigmentosa symptoms, including nyctalopia, constriction of the visual fields, and loss of central visual acuity, usually by the second decade of life. Belongs to the class-II aminoacyl-tRNA synthetase family. Protein type: Translation; EC 6.1.1.21; Ligase. Chromosomal Location of Human Ortholog: 5q31.3. Cellular Component: cytoplasm; cytosol. Molecular Function: histidine-tRNA ligase activity; ATP binding. Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; histidyl-tRNA aminoacylation. Disease: Usher Syndrome, Type Iiib

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

205

size3 :

1 mg

price3 :

1595

more info or order :

MyBioSource product webpage