protein

Recombinant Human Tissue Plasminogen Activator

MBS142404

0.02 mg

140 USD

Recombinant Protein

Recombinant Human Tissue Plasminogen Activator

Tissue Plasminogen Activator

tPA Human; Tissue Plasminogen Activator Human Recombinant; Tissue-type plasminogen activator; EC 3.4.21.68; tPA; t-PA; t-plasminogen activator; TPA; T-PA; DKFZp686I03148

tissue-type plasminogen activator isoform 1 preproprotein; Tissue-type plasminogen activator; tissue-type plasminogen activator; alteplase; plasminogen activator, tissue type; reteplase; t-plasminogen activator; tissue plasminogen activator (t-PA); plasminogen activator, tissue; INN: Alteplase; INN: ReteplaseCleaved into the following 2 chains:Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B

tPA

PLAT; PLAT; TPA; T-PA; t-PA; t-plasminogen activator; tPA

TPA_HUMAN

Chinese Hamster Ovarian Cells (CHO)

562

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Each mg of t-PA contains 1.7 gr L-arginine, 0.5 gr phosphoric acid and 4 mg tween 80. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized t-PA although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution tPA should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Enzymatic Activity: 580,000 IU/mg. Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder

Solubility: It is recommended to reconstitute the lyophilized t-PA in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

ENZYMES; Enzymes

Description: Tissue Plasminogen Activator Human Recombinant produced in CHO cells is a single, glycosylated polypeptide chain containing 527 amino acids and having a molecular mass of 59008.71 Dalton. tPA is a serine protease enzyme that converts plasminogen to plasmin. The tPA is purified by proprietary chromatographic techniques. Introduction: Tissue plasminogen activator (abbreviated PLAT or tPA) is a secreted serine proteasewhich converts the proenzymeplasminogento plasmin, a fibrinolyticenzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin.This enzyme plays a role in cell migrationand tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysiswhich can result in thrombosisor embolism.

4505861

NP_000921.1

NM_000930.3

P00750

44,373 Da

Blood Clotting Cascade Pathway (198840); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Dissolution Of Fibrin Clot Pathway (106061); Endochondral Ossification Pathway (198812); Hemostasis Pathway (106028); Orphan Transporters Pathway (1127546); Selenium Pathway (198825); Senescence And Autophagy Pathway (198780)

This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

PLAT: Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. Increased activity of TPA results in increased fibrinolysis of fibrin blood clots that is associated with excessive bleeding. Defective release of TPA results in hypofibrinolysis that can lead to thrombosis or embolism. Belongs to the peptidase S1 family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal; Vesicle; Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; EC 3.4.21.68; Protease. Chromosomal Location of Human Ortholog: 8p12. Cellular Component: extracellular matrix; extracellular space; cell surface; apical part of cell; cytoplasm; extracellular region; synapse; secretory granule. Molecular Function: protein binding; serine-type endopeptidase activity. Biological Process: negative regulation of proteolysis; synaptic transmission, glutamatergic; response to peptide hormone stimulus; fibrinolysis; response to cAMP; smooth muscle cell migration; response to hypoxia; regulation of synaptic plasticity; protein modification process; platelet-derived growth factor receptor signaling pathway; proteolysis; blood coagulation; transmembrane transport; plasminogen activation. Disease: Thrombophilia, Familial, Due To Decreased Release Of Tissue Plasminogen Activator

0.02 mg

140 USD

0.1 mg

245

1 mg

1075