Recombinant Mouse Fibroblast Growth Factor-21, His Tag | MyBioSource MBS142395 product information

product summary

request information :

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mouse Fibroblast Growth Factor-21, His Tag

catalog :

MBS142395

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142395

products type :

Recombinant Protein

products full name :

Recombinant Mouse Fibroblast Growth Factor-21, His Tag

products short name :

Fibroblast Growth Factor-21

products name syn :

FGF 21 Mouse, His; Fibroblast Growth Factor-21 Mouse Recombinant, His Tag; Fibroblast growth factor 21; FGF-21; mFGF 21 His

other names :

fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; FGF-21; fibroblast growth factor 21; N/A

products gene name :

mFGF 21

other gene names :

Fgf21; Fgf21; FGF-21

uniprot entry name :

FGF21_MOUSE

host :

E Coli

sequence length :

210

sequence :

MKHHHHHHAS AYPIPDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGAAHRSP ESLLELKALKPGVIQILGVK ASRFLCQQPD GALYGSPHFD PEACSFRELL LEDGYNVYQS EAHGLPLRLP QKDSPNQDATSWGPVRFLPM PGLLHEPQDQ AGFLPPEPPD VGSSDPLSMV EPLQGRSPSY AS.

purity :

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM TRIS, 20mM NaCl, pH 7.5. Filtered white lyophilized powder.

storage stability :

Lyophilized FGF-21 Mouse Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture. Add DTT (0.2mM) and NaCl (0.1-0.15M) before freezing to prevent potential aggregation.

products categories :

CYTOKINES AND GROWTH FACTORS; Growth Factors; Fibroblast Growth Factor

products description :

Description: Fibroblast Growth Factor -21 Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 192 amino acids and having a molecular mass of 21.2 kDa. The amino acid sequence of the recombinant human FGF21 is 100% homologous to the amino acid sequence of the Mouse FGF21 without signal sequence and contains 10 a.a. His tag at N-terminal.The FGF-21 is purified by proprietary chromatographic techniques. Introduction: The FGFs are a family of more than 20 small (~1726 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1, 2, or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response.FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.FGF-19, has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.

ncbi gi num :

9910218

ncbi acc num :

NP_064397.1

ncbi gb acc num :

NM_020013.4

uniprot acc num :

Q9JJN1

ncbi mol weight :

23,237 Da

ncbi pathways :

ESC Pluripotency Pathways 198374!!MAPK Signaling Pathway 83245!!MAPK Signaling Pathway 456!!Melanoma Pathway 83307!!Melanoma Pathway 526!!PI3K-Akt Signaling Pathway 692242!!PI3K-Akt Signaling Pathway 692979!!Pathways In Cancer 83298!!Rap1 Signaling Pathway 869018!!Rap1 Signaling Pathway 878042

size :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage