product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Chicken Leptin
catalog :
MBS142381
quantity :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142381
products type :
Recombinant Protein
products full name :
Recombinant Chicken Leptin
products short name :
Leptin
products name syn :
Leptin Chicken; Leptin Chicken Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; chLeptin
other names :
Leptin; Leptin; Obesity factor
other gene names :
LEP; OB
uniprot entry name :
LEP_CHICK
host :
E Coli
sequence length :
163
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Cys-Gln.
purity :
Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.19 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Chicken as a Reference Standard. Biological Activity: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity is however 5-10 fold lower as compared to mammalian leptins.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin
products description :
Description: Leptin Chicken Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 145 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic techniques. Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
ncbi gi num :
3024234
ncbi acc num :
O42164.1
uniprot acc num :
O42164
ncbi mol weight :
18,183 Da
size :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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