product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Porcine Leptin
catalog :
MBS142379
quantity :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142379
products type :
Recombinant Protein
products full name :
Recombinant Porcine Leptin
products short name :
Leptin
products name syn :
Leptin Porcine; Leptin Porcine Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; pLeptin
other names :
leptin; Leptin; leptin; obese; obesity factor; Obesity factor
other gene names :
LEP; LEP; OB; OBS; OB; OBS
uniprot entry name :
LEP_PIG
host :
E Coli
sequence length :
167
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Trp.
purity :
Greater than 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized recombinant porcine leptin in sterile 0.4% NaHCO3 adjusted, not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Porcine as a Reference Standard. Biological Activity: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin
products description :
Description: Leptin Porcine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and additional Ala at N-terminus, having a molecular mass of 16kDa. The Leptin is purified by proprietary chromatographic techniques. Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
ncbi gi num :
55741433
ncbi acc num :
NP_999005.1
ncbi gb acc num :
NM_213840.1
uniprot acc num :
Q29406
ncbi mol weight :
18,661 Da
ncbi pathways :
AMPK Signaling Pathway 989938!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 84504!!Adipocytokine Signaling Pathway 505!!Cytokine-cytokine Receptor Interaction Pathway 84463!!Cytokine-cytokine Receptor Interaction Pathway 460!!Incretin Synthesis, Secretion, And Inactivation Pathway 1125408!!Jak-STAT Signaling Pathway 84489!!Jak-STAT Signaling Pathway 488!!Metabolism Of Proteins Pathway 1125373
size :
0.02 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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