protein

Recombinant Bovine Leptin

MBS142378

0.02 mg

140 USD

Recombinant Protein

Recombinant Bovine Leptin

BLeptin

Leptin Bovine; Leptin Bovine Recombinant; OB Protein; Obesity Protein; OBS; Obesity factor; bLeptin

leptin; Leptin; leptin; leptin (obesity homolog, mouse); obese; obesity factor; leptin; Obesity factor

LEP; LEP; ob; OB; OBS

LEP_BOVIN

E Coli

167

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Arg.

Greater than 95.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

The protein was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Leptin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Leptin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.19 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC,using calibrated solution of Leptin Bovine as a Reference Standard. Biological Activity: Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin

Description: Leptin Bovine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16 kDa.The Leptin is purified by proprietary chromatographic techniques. Introduction: A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.

87196505

NP_776353.2

NM_173928.2

P50595

18,716 Da

AMPK Signaling Pathway (989258); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (84332); Adipocytokine Signaling Pathway (505); Adipogenesis Pathway (920913); Cytokine-cytokine Receptor Interaction Pathway (84290); Cytokine-cytokine Receptor Interaction Pathway (460); Incretin Synthesis, Secretion, And Inactivation Pathway (1119678); Jak-STAT Signaling Pathway (84316); Jak-STAT Signaling Pathway (488)

0.02 mg

140 USD

0.1 mg

205

1 mg

985