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Recombinant Human Stem Cell Factor, Sf9

MBS142297

0.01 mg

205 USD

MBS142297

Recombinant Protein

Recombinant Human Stem Cell Factor, Sf9

Stem Cell Factor

SCF Human, Sf9; Stem Cell Factor Human Recombinant, Sf9; Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250; SCF Sf9

kit ligand isoform b; Kit ligand; kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor; KIT ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligandSoluble KIT ligand; sKITLG

SCF

KITLG; KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7; MGF; SCF; MGF; SCF; sKITLG

SCF_HUMAN

Sf9 Insect Cells

273

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein is supplied in 1X PBS, pH 7.4. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution SCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Stem Cell Factor in 10 mM acetic acid not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is typically 2-5ng/ml, corresponding to a Specific Activity of 200,000-500,000units/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Stem Cell Factor

Description: Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is fused to a C-terminal His-tag (6x His) and purified by proprietary chromatographic techniques. Introduction: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases.SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

4505175

NP_000890.1

NM_000899.4

P21583

26,667 Da

Adaptive Immune System Pathway 366160!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 198811!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Disease Pathway 530764!!Downstream Signal Transduction Pathway 106385

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

0.01 mg

205 USD