product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Mouse Platelet Derived Growth Factor-BB
catalog :
MBS142288
quantity :
0.01 mg
price :
205 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142288
products type :
Recombinant Protein
products full name :
Recombinant Mouse Platelet Derived Growth Factor-BB
products short name :
Platelet Derived Growth Factor-BB
products name syn :
PDGF BB Mouse; Platelet-Derived Growth Factor BB Mouse Recombinant; Glioma-derived growth factor; GDGF; Osteosarcoma-derived Growth Factor; ODGF; SIS; SSV; PDGF2; c-sis; FLJ12858; PDGF-BB; PDGF B-chain; Platelet-derived growth factor beta polypeptide; Becaplermin
other names :
platelet-derived growth factor subunit B; Platelet-derived growth factor subunit B; platelet-derived growth factor subunit B; PDGF subunit B; PDGF-2; c-sis; platelet-derived growth factor B chain; platelet-derived growth factor beta polypeptide; proto-oncogene c-Sis; platelet derived growth factor, B polypeptide; PDGF-2; Platelet-derived growth factor B chain; Platelet-derived growth factor beta polypeptide; Proto-oncogene c-Sis
products gene name :
mPDGF BB
other gene names :
Pdgfb; Pdgfb; Sis; PDGF-B; Sis; PDGF subunit B
uniprot entry name :
PDGFB_MOUSE
host :
E Coli
sequence length :
241
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Leu-Gly-Ser.
purity :
Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized from 10mM NaCitrate pH-3.0. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution PDGF BB should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of PDGF-BB as a Reference Standard. Biological Activity: The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; PDGF
products description :
Description: Platelet-Derived Growth Factor BB Mouse Recombinant produced in E Coli, is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 (total of 2 chains 218aa) amino acids and having a total molecular weight of 24.6 kDa.PDGF-BB is purified by proprietary chromatographic techniques. Introduction: PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two splice variants have been identified for this gene.
ncbi gi num :
40538817
ncbi acc num :
NP_035187.2
ncbi gb acc num :
NM_011057.3
uniprot acc num :
P31240
ncbi mol weight :
27,382 Da
ncbi pathways :
Adaptive Immune System Pathway 1110669!!Choline Metabolism In Cancer Pathway 1060314!!Choline Metabolism In Cancer Pathway 1084232!!Constitutive PI3K/AKT Signaling In Cancer Pathway 1111361!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!DAP12 Interactions Pathway 1110771!!DAP12 Signaling Pathway 1110772!!Disease Pathway 1111319!!Downstream Signal Transduction Pathway 1110451
size :
0.01 mg
price :
205 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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