catalog number :
MBS142282
products type :
Recombinant Protein
products full name :
Recombinant Bovine Betacellulin
products short name :
BBetacellulin
products name syn :
BTC Bovine; Betacellulin Bovine Recombinant
other names :
Probetacellulin; Probetacellulin; probetacellulin; betacellulin
products gene name :
bBTC
other gene names :
BTC; BTC; BTC
uniprot entry name :
BTC_BOVIN
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr.
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The Betacellulin Bovine Recombinant was lyophilized after extensive dialysis against 50mM acetic acid. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Betacellulin Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution BTC Bovine should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized BTC Bovine in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.59 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of BTC as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependent proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 10.0 ng/ml, corresponding to a Specific Activity 100,000 units/mg.
products categories :
CYTOKINES AND GROWTH FACTORS; Cytokines; Betacellulin
products description :
Description: Betacellulin Bovine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 80 amino acids and having a molecular mass of 9003 Dalton. Betacellulin Bovine Recombinant is purified by proprietary chromatographic techniques. Introduction: Btc is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the egf receptor and other related receptors.
ncbi mol weight :
19,640 Da
ncbi pathways :
Adaptive Immune System Pathway (1119287); Constitutive PI3K/AKT Signaling In Cancer Pathway (1119781); DAP12 Interactions Pathway (1119396); DAP12 Signaling Pathway (1119397); Disease Pathway (1119688); Downstream Signal Transduction Pathway (1120118); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1119301); Downstream Signaling Of Activated FGFR Pathway (1119722); ErbB Signaling Pathway (84288); ErbB Signaling Pathway (920921)