catalog number :
MBS142271
products type :
Recombinant Protein
products full name :
Recombinant Rat Granulocyte Macrophage-Colony Stimulating Factor
products short name :
Granulocyte Macrophage-Colony Stimulating Factor
products name syn :
GM CSF Rat; Granulocyte Macrophage-Colony Stimulating Factor Rat Recombinant; CSF-2; MGI-1GM; GM-CSF; Pluripoietin-alpha; Molgramostin; Sargramostim; rGM CSF
other names :
granulocyte-macrophage colony-stimulating factor; Granulocyte-macrophage colony-stimulating factor; granulocyte-macrophage colony-stimulating factor; CSF; colony stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor; CSF
other gene names :
Csf2; Csf2; Gmcsf; Gm-csf; Csfgm; GM-CSF; CSF
uniprot entry name :
CSF2_RAT
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Arg.
purity :
Greater than 95.0% as determined by: (a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.
form :
GM-CSF Rat was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GMCSF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 range = 0.001-0.01ng/ml corresponding to a specific activity of 100,000,000-1,000,000,000IU/mg, determined by the dose dependent proliferation of murine MC/9 cells.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Colony Stimulating Factor
products description :
Description: Granulocyte Macrophage Colony Stimulating Factor Rat Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 128 amino acids and having a molecular mass of 14590.65 Dalton. GM-CSF Rat Recombinant is purified by proprietary chromatographic techniques. Introduction: GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
ncbi acc num :
NP_446304.1
ncbi gb acc num :
NM_053852.1
ncbi mol weight :
16,562 Da
ncbi pathways :
Amoebiasis Pathway 167311!!Amoebiasis Pathway 167191!!Cytokine Signaling In Immune System Pathway 1121619!!Cytokine-cytokine Receptor Interaction Pathway 83443!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response (BioCarta) Pathway 198521!!Fc Epsilon RI Signaling Pathway 83474!!Fc Epsilon RI Signaling Pathway 493!!G Beta:gamma Signalling Through PI3Kgamma Pathway 1120750!!G-protein Beta:gamma Signalling Pathway 1120749
ncbi summary :
plays a role in alveolar epithelial fluid transport [RGD, Feb 2006]