protein

Recombinant Rat Interleukin-12

MBS142267

0.002 mg

140 USD

Recombinant Protein

Recombinant Rat Interleukin-12

Interleukin-12

IL 12 Rat; Interleukin-12 Rat Recombinant; NKSF; CTL maturation factor (TCMF); Cytotoxic lymphocyte maturation factor (CLMF); TSF; Edodekin-alpha; IL-12

interleukin 12; Interleukin-12 subunit alpha; interleukin-12 subunit alpha; CLMF p35; IL-12, subunit p35; IL35 subunit; NF cell stimulatory factor chain 1; NK cell stimulatory factor chain 1; cytotoxic lymphocyte maturation factor 1, p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12, p35; interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35); interleukin-12 alpha chain; interleukin 12A; Cytotoxic lymphocyte maturation factor 35 kDa subunit; CLMF p35; IL-12 subunit p35; NK cell stimulatory factor chain 1; NKSF1

rIL 12

IL12A; IL12A; P35; CLMF; NFSK; NKSF1; IL-12A; NKSF1; IL-12A; CLMF p35; NKSF1

IL12A_HUMAN

Baculovirus

253

The sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.

Greater than 95.0% as determined by SDS-PAGE.

The protein (1mg/ml) was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Interleukin-12 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL12 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Interleukin 12 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: ED50 range = 0.01-0.05 ng/ml corresponding to a specific activity of 20,000,000-100,000,000IU/mg as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated mouse splenocytes that have been costimulated with PMA. Optimal concentration for individual application should be determined by a dose response assay.

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

Description: Interleukin-12 Rat Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 503 amino acids and having a molecular weight of 70 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques. Introduction: IL-12 is a heterodimeric cytokine that stimulates the production of interferon gamma from T-cells and natural killer cells, and also induces differentiation of Th1 helper cells. It is an initiator of cell-mediated immunity.

4323579

AAD16432.1

24,874 Da

African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine-cytokine Receptor Interaction Pathway (83051)

This gene encodes a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. [provided by RefSeq, Jul 2008]

IL12A: Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine- activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. Belongs to the IL-6 superfamily. Protein type: Secreted; Cytokine; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q25.33. Cellular Component: extracellular space; interleukin-12 complex; cytoplasm. Molecular Function: protein binding; interleukin-27 binding; interleukin-12 beta subunit binding; growth factor activity; protein heterodimerization activity; cytokine activity; interleukin-12 receptor binding. Biological Process: positive regulation of NK T cell activation; cell migration; positive regulation of cell adhesion; positive regulation of natural killer cell mediated cytotoxicity directed against tumor cell target; negative regulation of smooth muscle cell proliferation; positive regulation of T cell mediated cytotoxicity; response to virus; positive regulation of tyrosine phosphorylation of Stat4 protein; response to lipopolysaccharide; positive regulation of natural killer cell mediated cytotoxicity; defense response to protozoan; positive regulation of natural killer cell activation; response to UV-B; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; negative regulation of interleukin-17 production; positive regulation of T cell differentiation; positive regulation of mononuclear cell proliferation; positive regulation of lymphocyte proliferation; immune response; positive regulation of T cell proliferation; cell cycle arrest

0.002 mg

140 USD

0.01 mg

205

0.1 mg

1030