product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Mouse Fibroblast Growth Factor-basic
catalog :
MBS142263
quantity :
0.01 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142263
products type :
Recombinant Protein
products full name :
Recombinant Mouse Fibroblast Growth Factor-basic
products short name :
Fibroblast Growth Factor-basic
products name syn :
FGF 2 Mouse; Fibroblast Growth Factor-Basic Mouse Recombinant; HBGH-2; HBGF-2; Prostatropin; FGF-2; FGB-b
other names :
fibroblast growth factor 2; Fibroblast growth factor 2; fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2; fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
products gene name :
mFGF 2
other gene names :
Fgf2; Fgf2; Fgfb; bFGF; Fgf-2; Fgf-2; FGF-2; bFGF; HBGF-2
uniprot entry name :
FGF2_MOUSE
host :
E Coli
sequence length :
154
sequence :
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS.
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
FGF-b was lyophilized from 5mM Na2PO4, pH7.5 and 50mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGF-basic should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor b in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard. Biological Activity: The activity as calculated by the dose-dependant proliferation of BALB/3T3 cells was found to be 0.1-0.16ng/ml.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Fibroblast Growth Factor
products description :
Description: Fibroblast Growth Factor-basic Mouse Recombinant (FGF-2) produced in E Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.3kDa. The FGF-2 is purified by proprietary chromatographic techniques. Introduction: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.
ncbi gi num :
7106315
ncbi acc num :
NP_032032.1
ncbi gb acc num :
NM_008006.2
uniprot acc num :
P15655
ncbi mol weight :
17,153 Da
ncbi pathways :
Activated Point Mutants Of FGFR2 Pathway 1111339!!Adaptive Immune System Pathway 1110669!!Constitutive PI3K/AKT Signaling In Cancer Pathway 1111361!!DAP12 Interactions Pathway 1110771!!DAP12 Signaling Pathway 1110772!!Disease Pathway 1111319!!Downstream Signal Transduction Pathway 1110451!!Downstream Signaling Events Of B Cell Receptor (BCR) Pathway 1110683!!Downstream Signaling Of Activated FGFR Pathway 1110382!!ESC Pluripotency Pathways 198374
size :
0.01 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
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