protein

Recombinant Rat Interleukin-2

MBS142259

0.005 mg

140 USD

Recombinant Protein

Recombinant Rat Interleukin-2

[Interleukin-2]

[IL 2 Rat; Interleukin-2 Rat Recombinant; T-cell growth factor (TCGF); Aldesleukin; Lymphokine; IL-2]

[interleukin-2; Interleukin-2; interleukin-2; IL-2; T-cell growth factor; TCGF; interleukin 2; T-cell growth factor; TCGF]

[rIL 2]

[Il2; Il2; Il-2; IL-2; TCGF]

IL2_RAT

E Coli

155

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Pro-Thr-Ser

Greater than 95.0% as determined by SDS-PAGE.

The protein (1mg/ml) was lyophilized without any additives.

Lyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18° C. Upon reconstitution IL2 should be stored at 4° C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Physical Appearance: Sterile Filtered White Lyophilized (freeze-dried) powder.

Solubility: It is recommended to reconstitute the lyophilized Interleukin 2 in sterile 100mM AcOH not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:. 1. UV spectroscopy at 280 nm using the absorbency value of 0.535 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-2 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine CTLL-2 cells is 0.1-0.4 ng/ml corresponding to a specific activity of 2,500,000-10,000,000 units/mg.

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

Description: Interleukin-2 Rat Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 17kDa. The IL-2 is purified by proprietary chromatographic techniques. Introduction: IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

16758692

NP_446288.1

NM_053836.1

P17108

17,633 Da

Allograft Rejection Pathway (83511); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83509); Autoimmune Thyroid Disease Pathway (533); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (1121619); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198521)

cytokine produced by T-cells in response to antigen or mitogen stimulation [RGD, Feb 2006]

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

0.005 mg

140 USD

0.02 mg

205

1 mg

2475