Recombinant Mouse Interleukin-13 | MyBioSource MBS142252 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Mouse Interleukin-13

catalog :

MBS142252

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142252

products type :

Recombinant Protein

products full name :

Recombinant Mouse Interleukin-13

products short name :

Interleukin-13

products name syn :

IL 13 Mouse; Interleukin-13 Mouse Recombinant; Interleukin-13; NC300; ALRH; BHR1; P600; IL-13; IL13

other names :

interleukin-13; Interleukin-13; interleukin-13; T-cell activation protein P600; interleukin 13; T-cell activation protein P600

products gene name :

mIL 13

other gene names :

Il13; Il13; Il-13; Il-13; IL-13

uniprot entry name :

IL13_MOUSE

host :

E Coli

sequence length :

131

sequence :

MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F.

purity :

Greater than 95% as determined by SDS-PAGE.

form :

The protein (1mg/ml) was lyophilized in PBS, pH 7.2 and 5% trehalose. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL13 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.69 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard. Biological Activity: The ED50 range=4 ng/ml, corresponding to a specific activity of 250,000IU/mg as determined by the dose dependent proliferation of TF-1 cells.

products categories :

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

products description :

Description: Interleukin-13 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 111 amino acids and having a molecular mass of 12.3 kDa. The IL-13 is purified by proprietary chromatographic techniques. Introduction: IL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

ncbi gi num :

6680403

ncbi acc num :

NP_032381.1

ncbi gb acc num :

NM_008355.3

uniprot acc num :

P20109

ncbi mol weight :

14,108 Da

ncbi pathways :

Asthma Pathway (83313); Asthma Pathway (532); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Cytokines And Inflammatory Response (BioCarta) Pathway (198283); Fc Epsilon RI Signaling Pathway (83279); Fc Epsilon RI Signaling Pathway (493); Inflammatory Bowel Disease (IBD) Pathway (842778); Inflammatory Bowel Disease (IBD) Pathway (842797); Jak-STAT Signaling Pathway (83274)

uniprot summary :

IL13: Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Defects in IL13 may be a cause of susceptibility to allergic rhinitis (ALRH). Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure. Belongs to the IL-4/IL-13 family. Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted. Cellular Component: extracellular space; cytoplasm; extracellular region; external side of plasma membrane. Molecular Function: interleukin-13 receptor binding; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity. Biological Process: response to nicotine; positive regulation of smooth muscle cell proliferation; regulation of proton transport; microglial cell activation; positive regulation of mast cell degranulation; positive regulation of connective tissue growth factor production; positive regulation of tyrosine phosphorylation of Stat6 protein; positive regulation of immunoglobulin production; positive regulation of protein secretion; positive regulation of B cell proliferation; positive regulation of ion transport; immune response; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; negative regulation of NAD(P)H oxidase activity; positive regulation of macrophage activation

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4550

more info or order :

MyBioSource product webpage