catalog number :
MBS142249
products type :
Recombinant Protein
products full name :
Recombinant Mouse Interleukin-7
products short name :
Interleukin-7
products name syn :
IL 7 Mouse; Interleukin-7 Mouse Recombinant; Lymphopoietin 1 (LP-1); pre-B cell factor; IL-7
other names :
interleukin-7; Interleukin-7; interleukin-7; interleukin 7; N/A
products gene name :
mIL 7
other gene names :
Il7; Il7; Il-7; hlb368; A630026I06Rik; Il-7; IL-7
uniprot entry name :
IL7_MOUSE
sequence :
MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI.
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Filtered (0.2 um) and lyophilized from a concentrated (1mg/ml) solution in 1xPBS, pH7.4. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL7 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.48 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine 2E8 cells is < 0.2ng/ml, corresponding to a Specific Activity of 5,000,000 units/mg.
products categories :
CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin
products description :
Description: Interleukin-7 Mouse Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 130 amino acids and having a molecular mass of 15.0 kDa. The IL-7 is purified by proprietary chromatographic techniques. Introduction: Interleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages.
ncbi acc num :
NP_032397.1
ncbi gb acc num :
NM_008371.4
ncbi mol weight :
17,727 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway 1110778!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response (BioCarta) Pathway 198283!!Hematopoietic Cell Lineage Pathway 83275!!Hematopoietic Cell Lineage Pathway 489!!Immune System Pathway 1110668!!Interleukin-7 Signaling Pathway 1110794!!Jak-STAT Signaling Pathway 83274!!Jak-STAT Signaling Pathway 488