product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Human Epidermal Growth Factor, Pichia
catalog :
MBS142209
quantity :
0.1 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142209
products type :
Recombinant Protein
products full name :
Recombinant Human Epidermal Growth Factor, Pichia
products short name :
Epidermal Growth Factor
products name syn :
EGF Human, Pichia; Epidermal Growth Factor Human Recombinant, Pichia; Urogastrone; URG; EGF; EGF Pichia
other names :
Epidermal growth factor; Epidermal growth factor; Epidermal growth factor
products gene name :
EGF
other gene names :
EGF; N/A
uniprot entry name :
Q6QBS2_HUMAN
host :
Pichia Pastoris
sequence length :
53
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Asp-Ser-Glu, which agrees with the sequence of native EGF human.N-terminal methionine has been completely removed enzymatically.
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Each lyophilized mg contains 0.15M NaCl, 0.025M sodium bicarbonate, pH-7.5. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution EGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000Units/mg.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Epidermal Growth Factor
products description :
Description: Epidermal Growth Factor Human Recombinant produced in Pichia Pastoris is a single, glycosylated, polypeptide chain containing 51 amino acids and having a molecular mass of 6KDa.The EGF is purified by proprietary chromatographic techniques. Introduction: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
ncbi gi num :
74749288
ncbi acc num :
Q6QBS2
uniprot acc num :
Q6QBS2
ncbi mol weight :
6,222 Da
size :
0.1 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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