product summary
request information :
company name :
MyBioSource
product type :
protein
product name :
Recombinant Mouse Epidermal Growth Factor
catalog :
MBS142203
quantity :
0.1 mg
price :
140 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142203
products type :
Recombinant Protein
products full name :
Recombinant Mouse Epidermal Growth Factor
products short name :
Epidermal Growth Factor
products name syn :
EGF Mouse; Epidermal Growth Factor Mouse Recombinant; Urogastrone; URG; EGF
other names :
pro-epidermal growth factor; Pro-epidermal growth factor; pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); epidermal growth factor; N/A
products gene name :
mEGF
other gene names :
Egf; Egf; AI790464; EGF
uniprot entry name :
EGF_MOUSE
host :
E Coli
sequence length :
1217
sequence :
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-Tyr-Pro-Gly, which agrees with the sequence of native Epidermal Growth Factor human.
purity :
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The protein was lyophilized from a concentrated (1mg/ml) solution containing 1x PBS pH-7.4. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution EGF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 3 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000 units/mg.
products categories :
CYTOKINES AND GROWTH FACTORS; Growth Factors; Epidermal Growth Factor
products description :
Description: Epidermal Growth Factor Mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids including 3 intramolecular disulfide-bonds and having a molecular mass of 6 kDa.The EGF is purified by proprietary chromatographic techniques. Introduction: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
ncbi gi num :
170172524
ncbi acc num :
NP_034243.2
ncbi gb acc num :
NM_010113.3
uniprot acc num :
P01132
ncbi mol weight :
133,072 Da
ncbi pathways :
Adaptive Immune System Pathway 1110669!!Bladder Cancer Pathway 83308!!Bladder Cancer Pathway 527!!Choline Metabolism In Cancer Pathway 1060314!!Choline Metabolism In Cancer Pathway 1084232!!Constitutive PI3K/AKT Signaling In Cancer Pathway 1111361!!Constitutive Signaling By EGFRvIII Pathway 1111328!!Constitutive Signaling By Ligand-Responsive EGFR Cancer Variants Pathway 1111326!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460
size :
0.1 mg
price :
140 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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