protein

Recombinant Human Visfatin

MBS142195

1 mg

4045 USD

Recombinant Protein

Recombinant Human Visfatin

Visfatin

Visfatin Human; Visfatin Human Recombinant; PBEF; Pre-B cell colony-enhancing factor; Nicotinamide phosphoribosyltransferase NAmPRTase; Nampt; MGC117256; DKFZP666B131; 1110035O14Rik; Visfatin

nicotinamide phosphoribosyltransferase; Nicotinamide phosphoribosyltransferase; nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1; nicotinamide phosphoribosyltransferase; Pre-B-cell colony-enhancing factor 1; Pre-B cell-enhancing factor; Visfatin

NAMPT; NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik; PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor

NAMPT_HUMAN

E Coli

491

MPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQEIV EGMKQKMWSI ENIAFGSGGG LLQKLTRDLL NCSFKCSYVV TNGLGINVFK DPVADPNKRS KKGRLSLHRT PAGNFVTLEE GKGDLEEYGQ DLLHTVFKNG KVTKSYSFDE IRKNAQLNIE LEAAHH

Greater than 95.0% as determined by SDS-PAGE.

Visfatin was lyophilized with no additives. Filtered White lyophilized (freeze-dried) powder.

Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Visfatin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Wait several minutes for full reconstitution and solubility. Biological Activity: The activity is determined by its ability to induce IL-6, IL-1 beta and TNF alpha production from human PBMCs at 100ng/ml.

CYTOKINES AND GROWTH FACTORS; Cytokines; Visfatin

Description: Visfatin Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 466 amino acids. The total molecular mass is 52.6kDa (calculated). The Visfatin is purified by Flag-affinity chromatography. Introduction: Excess adiposity is the most important risk in the development of insulin resistance and type 2 diabetes mellitus (T2DM). Adipose tissue produces several proteins (adipocytokines) such as leptin, adiponectin, resistin, tumor necrosis factor-?, and IL-6, that modulate insulin sensitivity and appear to play an important role in the pathogenesis of insulin resistance, diabetes, dyslipidemia, inflammation, and atherosclerosis. However, the mechanisms by which fat tissue induces insulin resistance and the role of adipocytokines in the pathogenesis of T2DM have not been well established. Visfatin, also known as pre-B cell colony-enhancing factor (PBEF), is a cytokine that is highly expressed in visceral fat and was originally isolated as a secreted factor that synergizes with IL-7 and stem cell factors to promote the growth of B cell precursors. Visfatin homologs have been identified in carp, invertebrate mollusks, and bacteria, as well as in vertebrates, including humans and the mouse. It has been postulated to play a role in innate immunity.Visfatin exerts insulin-mimetic effects that are dose-dependent and quantitatively similar to those of insulin in stimulating muscle and adipocyte glucose transport, and in inhibiting hepatocyte glucose production. Intravenous injection of recombinant visfatin in mice decreased plasma glucose in a dose-dependent fashion. In keeping with its insulin-mimetic effects, visfatin was as effective as insulin in reducing hyperglycemia in insulin-deficient diabetic mice. Visfatin was also found to be bound to and activate insulin receptor, causing receptor phosphorylation and the activation of downstream signaling molecules. However, visfatin and insulin did not compete for binding to the insulin receptor, indicating that the two proteins were recognized by different regions of the receptor. Thus, visfatin might play a role in glucose homeostasis and dysregulation in biosynthesis or signal transduction, and might contribute to the pathogenesis of diabetes.

5031977

NP_005737.1

NM_005746.2

P43490

55,521 Da

Adipogenesis Pathway 198832!!BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway 477138!!Circadian Clock Pathway 187173!!Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway 906000!!Defective BTD Causes Biotidinase Deficiency Pathway 906015!!Defective CD320 Causes Methylmalonic Aciduria Pathway 906012!!Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway 906001!!Defective GIF Causes Intrinsic Factor Deficiency Pathway 906004!!Defective HLCS Causes Multiple Carboxylase Deficiency Pathway 906014!!Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway 906003

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

1 mg

4045 USD