protein

Recombinant Human Glial-Derived Neurotrophic Factor

MBS142182

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Glial-Derived Neurotrophic Factor

Glial-Derived Neurotrophic Factor

GDNF Human; Glial-Derived Neurotrophic Factor Human Recombinant; ATF1; ATF2; HFB1-GDNF; GDNF

glial cell line-derived neurotrophic factor isoform 1 preproprotein; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; glial cell derived neurotrophic factor; Astrocyte-derived trophic factor; ATF

GDNF

GDNF; GDNF; ATF1; ATF2; HSCR3; HFB1-GDNF; hGDNF; ATF

GDNF_HUMAN

E Coli

211

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Pro-Asp-Lys.

Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

GDNF was lyophilized after dialysis against 10mM sodium citrate and 150mM NaCl. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GDNF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50, calculated by the dose-dependant dopamin uptake in rat mesencephalic cultures was found to be 5-10 ng/ml, corresponding to a specific activity of 100,000-200,000Units/mg.

CYTOKINES AND GROWTH FACTORS; Neurotrophins; GDNF

Description: Glial derived Neurotrophic Factor Human Recombinant produced in E Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 135 amino acids and having a total molecular mass of 30,360 Dalton. GDNF is purified by proprietary chromatographic techniques. Introduction: GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

4503975

NP_000505.1

NM_000514.3

P39905

18,123 Da

Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); NCAM Signaling For Neurite Out-growth Pathway (105689); NCAM1 Interactions Pathway (105697); Signaling Events Regulated By Ret Tyrosine Kinase Pathway (137915); Spinal Cord Injury Pathway (739007)

This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene may be associated with Hirschsprung disease. [provided by RefSeq, Jun 2010]

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 5p13.1-p12. Cellular Component: extracellular region. Molecular Function: protein homodimerization activity; growth factor activity; receptor binding. Biological Process: nervous system development; positive regulation of dopamine secretion; axon guidance; peristalsis; adult locomotory behavior; mRNA stabilization; positive regulation of monooxygenase activity; regulation of dopamine uptake; signal transduction; enteric nervous system development; sympathetic nervous system development; regulation of gene expression; ureteric bud branching; induction of an organ; positive regulation of cell proliferation; postganglionic parasympathetic nervous system development; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; postsynaptic membrane organization; positive regulation of cell differentiation; metanephros development; neural crest cell migration; neurite development; negative regulation of apoptosis. Disease: Hirschsprung Disease, Susceptibility To, 3; Central Hypoventilation Syndrome, Congenital; Pheochromocytoma

0.002 mg

140 USD

0.01 mg

205

1 mg

4550