protein

Recombinant Bovine Fibroblast Growth Factor-basic

MBS142165

0.01 mg

140 USD

Recombinant Protein

Recombinant Bovine Fibroblast Growth Factor-basic

BFibroblast Growth Factor-basic

FGF 2 Bovine; Fibroblast Growth Factor-Basic Bovine Recombinant; HBGH-2; HBGF-2; Prostatropin; FGF-2; FGB-b

fibroblast growth factor 2; Fibroblast growth factor 2; fibroblast growth factor 2; BFGF; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2; fibroblast growth factor 2 (basic); Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

bFGF 2

FGF2; FGF2; FGF-2; FGF-2; bFGF; HBGF-2

FGF2_BOVIN

E Coli

155

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The FGF-b Bovine was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% HSA. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. Analysis by RP-HPLC, using a calibrated solution of FGF-2 Bovine as a Reference Standard. Biological Activity: The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1ng/ml, corresponding to a specific activity of 3 x 106 Units/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Fibroblast Growth Factor

Description: FGF-2 Bovine Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques. Introduction: FGF-basic is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. there are differences in the tissue distribution and concentration of these 2 growth factors.

27806627

NP_776481.1

NM_174056.3

P03969

17,250 Da

Activated Point Mutants Of FGFR2 Pathway (1119735); Adaptive Immune System Pathway (1119287); Constitutive PI3K/AKT Signaling In Cancer Pathway (1119781); DAP12 Interactions Pathway (1119396); DAP12 Signaling Pathway (1119397); Disease Pathway (1119688); Downstream Signal Transduction Pathway (1120118); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1119301); Downstream Signaling Of Activated FGFR Pathway (1119722); Endochondral Ossification Pathway (920938)

0.01 mg

140 USD

0.05 mg

205

1 mg

1285