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protein

Recombinant Human Leptin, His Tag

MBS142164

1 mg

2365 USD

MBS142164

Recombinant Protein

Recombinant Human Leptin, His Tag

Leptin

Leptin Human, His; Leptin Human Recombinant, His Tag; OB Protein; Obesity Protein; OBS; Obesity factor; Leptin His

leptin; Leptin; leptin; leptin (murine obesity homolog); leptin (obesity homolog, mouse); obese protein; obese, mouse, homolog of; obesity factor; leptin; Obese protein; Obesity factor

LEP; LEP; OB; OBS; LEPD; OB; OBS

LEP_HUMAN

E Coli

167

Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein was lyophilized from a concentrated (1mg/ml) solution with 1X PBS, 0.1% SDS and 1mM DTT. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Leptin although stable at room temperature, should be stored desiccated below 0 degree C. Reconstituted Leptin is best stored refrigerated at 4 degree C.Please avoid freeze-thaw cycles.

Western Blot (WB), Antibody Production, Protein assay

1. Positive control for Western blot.2. Antibody production. 3. Protein assay.

Solubility: The lyophilized Leptin is very soluble in water and most aqueous buffers below and above the isoelectric point.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Leptin

Description: Leptin Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing amino acids 48-167 and having a total molecular mass of 19 kDa including the 4 kDa His tag.The Leptin is purified by proprietary chromatographic techniques. Introduction: Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16 kDa in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells.

4557715

NP_000221.1

NM_000230.2

P41159

18,641 Da

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!Adipocytokine Signaling Pathway 83093!!Adipocytokine Signaling Pathway 505!!Adipogenesis Pathway 198832!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Developmental Biology Pathway 477129!!HIF-1-alpha Transcription Factor Network Pathway 138045

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

1 mg

2365 USD