protein

Recombinant Human Fibroblast Growth Factor-21, His Tag

MBS142158

0.01 mg

140 USD

Recombinant Protein

Recombinant Human Fibroblast Growth Factor-21, His Tag

Fibroblast Growth Factor-21

FGF 21 Human, His; Fibroblast Growth Factor-21 Human Recombinant, His Tag; Fibroblast growth factor 21; FGF-21; FGF 21 His

fibroblast growth factor 21; Fibroblast growth factor 21; fibroblast growth factor 21; fibroblast growth factor 21

FGF 21

FGF21; FGF21; FGF-21

FGF21_HUMAN

E Coli

209

MGSSHHHHHH SSGLVPRGSH MHPIPDSSPL LQFGGQVRQR YLYTDDAQQT EAHLEIREDG TVGGAADQSP ESLLQLKALK PGVIQILGVKTSRFLCQRPD GALYGSLHFD PEACSFRELL LEDGYNVYQS EAHGLPLHLP GNKSPHRDPA PRGPARFLPL PGLPPAPPEP PGILAPQPPD VGSSDPLSMV GPSQGRSPSY AS.

Greater than 90.0% as determined by SDS-PAGE.

The FGF-21 His tag protein solution in 20mM Tris-HCL buffer pH-8 and 10% glycerol. Sterile Filtered colorless clear solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Please avoid freeze thaw cycles.

Biological Activity: Measured in a cell proliferation assay using NIH-3T3 cells. The ED50 for this effect is 83,000 units/ml.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Fibroblast Growth Factor

Description: Fibroblast Growth Factor -21 Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 202 amino acids (29-209) and having a molecular mass of 21.6 kDa. The FGF-21 is fused to 20 amino acid His Tga at N-terminus and purified by proprietary chromatographic techniques. Introduction: The FGFs are a family of more than 20 small (~1726 kDa) secreted peptides. The initial characterization of these proteins focused on their ability to stimulate fibroblast proliferation. This mitogenic activity was mediated through FGF receptors (FGFRs) 1, 2, or 3. A fourth closely related tyrosine kinase receptor (FGFR4) was able to bind the FGFs but did not lead to a mitogenic response.FGFs modulate cellular activity via at least 5 distinct subfamilies of high-affinity FGF receptors (FGFRs): FGFR-1, -2, -3, and -4, all with intrinsic tyrosine kinase activity and, except for FGFR-4, multiple splice isoforms, and FGFR-5, which lacks an intracellular kinase domain. There is growing evidence that FGFRs can be important for regulation of glucose and lipid homeostasis. The overexpression of a dominant negative form of FGFR-1 in ? cells leads to diabetes in mice, which thus implies that proper FGF signaling is required for normal ? cell function and glycemia maintenance. FGFR-2 appears to be a key molecule during pancreatic development. Moreover, FGFR-4 has been implicated in cholesterol metabolism and bile acid synthesis.FGF-19, has been shown to cause resistance to diet-induced obesity and insulin desensitization and to improve insulin, glucose, and lipid profiles in diabetic rodents. Since these effects, at least in part, are mediated through the observed changes in metabolic rates, FGF-19 can be considered as a regulator of energy expenditure.FGF-21 is preferentially expressed in liver, but an exact knowledge of FGF-21 bioactivity and its mode of action have been lacking to date. FGF-21 is a potent activator of glucose uptake on adipocytes, protects animals from diet-induced obesity when overexpressed in transgenic mice, and lowers blood glucose and triglyceride levels when therapeutically administered to diabetic rodents.

9506597

NP_061986.1

NM_019113.2

Q9NSA1

22,300 Da

MAPK Signaling Pathway 83048!!MAPK Signaling Pathway 456!!Melanoma Pathway 83114!!Melanoma Pathway 526!!PI3K-Akt Signaling Pathway 692234!!PI3K-Akt Signaling Pathway 692979!!Pathways In Cancer 83105!!Rap1 Signaling Pathway 868086!!Rap1 Signaling Pathway 878042!!Ras Signaling Pathway 868085

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined. [provided by RefSeq, Jul 2008]

0.01 mg

140 USD