Recombinant Human Ciliary Neurotrophic Factor | MyBioSource MBS142149 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Ciliary Neurotrophic Factor

catalog :

MBS142149

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142149

products type :

Recombinant Protein

products full name :

Recombinant Human Ciliary Neurotrophic Factor

products short name :

Ciliary Neurotrophic Factor

products name syn :

CNTF Human; Ciliary-Neurotrophic Factor Human Recombinant; HCNTF; CNTF; Ciliary Neurotrophic Factor

other names :

ciliary neurotrophic factor; Ciliary neurotrophic factor; ciliary neurotrophic factor; ciliary neurotrophic factor

products gene name :

CNTF

other gene names :

CNTF; CNTF; HCNTF; CNTF

uniprot entry name :

CNTF_HUMAN

host :

E Coli

sequence length :

200

sequence :

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Phe-Thr-Glu.

purity :

Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

Lyophilized from a concentrated (1mg/ml) solution in water containing 5mM sodium Phosphate buffer pH=7.5 and 5mM sodium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Ciliary Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CNTF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized HCNTF in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: CNTF quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 1.28 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of CNTF Recombinant as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Neurotrophins; Ciliary-Neurotrophic Factor

products description :

Description: Ciliary Neurotrophic Factor Recombinant Human produced in E Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and having a molecular mass of 22706 Dalton. The CNTF is purified by proprietary chromatographic techniques. Introduction: CNTF is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. In addition to the predominant monocistronic transcript originating from this locus, the gene is also co-transcribed with the upstream ZFP91 gene. Co-transcription from the two loci results in a transcript that contains a complete coding region for the zinc finger protein but lacks a complete coding region for ciliary neurotrophic factor.CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

ncbi gi num :

4758020

ncbi acc num :

NP_000605.1

ncbi gb acc num :

NM_000614.3

uniprot acc num :

P26441

ncbi mol weight :

22,931 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Delta-Notch Signaling Pathway (198879); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488)

ncbi summary :

The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of the upstream ZFP91 gene and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse. [provided by RefSeq, Oct 2010]

uniprot summary :

CNTF: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy. Belongs to the CNTF family. Chromosomal Location of Human Ortholog: 11q12.2. Cellular Component: extracellular space; cytoplasm. Molecular Function: interleukin-6 receptor binding; growth factor activity; ciliary neurotrophic factor receptor binding. Biological Process: negative regulation of photoreceptor cell differentiation; positive regulation of cell proliferation; neuron development; regulation of retinal cell programmed cell death; negative regulation of neuron apoptosis; signal transduction; muscle morphogenesis; positive regulation of tyrosine phosphorylation of Stat3 protein; growth

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

2665

more info or order :

MyBioSource product webpage