protein

Recombinant Human Insulin Like Growth Factor-2

MBS142142

0.01 mg

140 USD

Recombinant Protein

Recombinant Human Insulin Like Growth Factor-2

Insulin Like Growth Factor-2

IGF2 Human; Insulin-Like Growth Factor-2 Human Recombinant; Somatomedin-A; IGF2; INSIGF; pp9974; C11orf43; FLJ22066; FLJ44734

insulin-like growth factor II isoform 1 preproprotein; Insulin-like growth factor II; insulin-like growth factor II; T3M-11-derived growth factor; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor type 2; insulin-like growth factor 2; Somatomedin-A; T3M-11-derived growth factorCleaved into the following 3 chains:Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin

IGF2

IGF2; IGF2; IGF-II; PP9974; C11orf43; IGF-II

IGF2_HUMAN

E Coli

180

AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE.

Greater than 97.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized IGF-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGFII should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized IGF2 in sterile 18M Omega -cm H2O at a concentration of 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50, calculated by the dose-dependent induction of MCF-7 cell proliferation was found to be 0.85ng/ml corresponding to a Specific Activity of 1,176,470IU/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Insulin-Like Growth Factor

Description: Insulin-Like Growth Factor-II Human Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 67 amino acids and having a molecular mass of 7505 Dalton. IGF-II is purified by proprietary chromatographic techniques. Introduction: IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.

4504609

NP_000603.1

NM_000612.5

P01344

26,331 Da

Apoptosis Pathway (198797); Cardiac Progenitor Differentiation Pathway (712094); Endochondral Ossification Pathway (198812); Hedgehog Signaling Pathway (198835); Hemostasis Pathway (106028); IGF1R Signaling Cascade Pathway (730346); IRS-related Events Triggered By IGF1R Pathway (730348); Metabolism Of Proteins Pathway (106230); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050)

This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: extracellular space; extracellular region; plasma membrane. Molecular Function: protein serine/threonine kinase activator activity; insulin-like growth factor receptor binding; protein binding; growth factor activity; hormone activity; receptor activator activity; insulin receptor binding. Biological Process: positive regulation of catalytic activity; response to nicotine; genetic imprinting; multicellular organismal development; positive regulation of glycogen biosynthetic process; exocrine pancreas development; female pregnancy; positive regulation of activated T cell proliferation; response to estradiol stimulus; response to radiation; platelet degranulation; positive regulation of MAPKKK cascade; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; skeletal development; response to drug; response to nutrient levels; platelet activation; positive regulation of mitosis; glucose metabolic process; memory; osteoblast differentiation; positive regulation of protein kinase B signaling cascade; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; cellular protein metabolic process; insulin receptor signaling pathway; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of insulin receptor signaling pathway; positive regulation of protein amino acid phosphorylation; blood coagulation. Disease: Silver-russell Syndrome; Growth Restriction, Severe, With Distinctive Facies; Beckwith-wiedemann Syndrome; Wilms Tumor 1

0.01 mg

140 USD

0.05 mg

205

1 mg

1285