protein

Recombinant Human Neurotrophin-3

MBS142134

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Neurotrophin-3

Neurotrophin-3

NT 3 Human; Neurotrophin-3 Human Recombinant; Neurotrophic factor; Nerve growth factor-2; NGF-2; HDNF; NT-3

neurotrophin-3 isoform 1 preproprotein; Neurotrophin-3; neurotrophin-3; nerve growth factor 2; neurotrophic factor; neurotrophin 3; HDNF; Nerve growth factor 2; NGF-2; Neurotrophic factor

NT 3

NTF3; NTF3; NT3; HDNF; NGF2; NT-3; NGF-2; NT-3; NGF-2

NTF3_HUMAN

E Coli

270

YAEHKSHRGE YSVCDSESLW VTDKSSAIDI RGHQVTVLGE IKTGNSPVKQ YFYETRCKEA RPVKNGCRGI DDKHWNSQCK TSQTYVRALT SENNKLVGWR WIRIDTSCVC ALSRKIGRT.

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized NGF2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution NGF-2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 2.165 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of NT-3 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50ng/ml, corresponding to a Specific Activity of 20,000-50,000IU/mg.

CYTOKINES AND GROWTH FACTORS; Neurotrophins

Description: Neurotrophin-3 Human Recombinant produced in E Coli is a non-glycosylated and non-covalently linked homodimer, containing 2x119 amino acid chains, having a total Mw of 27.2 kDa.The NT-3 is purified by proprietary chromatographic techniques. Introduction: NT3 a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.

156630995

NP_001096124.1

NM_001102654.1

P20783

30,800 Da

BDNF Signaling Pathway (712093); Integrated Pancreatic Cancer Pathway (711360); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Neurotrophic Factor-mediated Trk Receptor Signaling Pathway (137977); Neurotrophin Signaling Pathway (101143); Neurotrophin Signaling Pathway (100064); Trk Receptor Signaling Mediated By The MAPK Pathway (138044); P75(NTR)-mediated Signaling Pathway (138019)

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide; Cytokine. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: cytoplasmic membrane-bound vesicle; extracellular region. Molecular Function: neurotrophin p75 receptor binding; growth factor activity; nerve growth factor binding; receptor binding; chemoattractant activity. Biological Process: myelination; axon guidance; mechanoreceptor differentiation; epidermis development; glial cell fate determination; regulation of neuron differentiation; activation of MAPK activity; positive regulation of receptor internalization; positive regulation of glial cell differentiation; signal transduction; enteric nervous system development; induction of positive chemotaxis; positive chemotaxis; cell-cell signaling; positive regulation of cell proliferation; negative regulation of neuron apoptosis; regulation of synaptic transmission; nervous system development; negative regulation of peptidyl-tyrosine phosphorylation; activation of protein kinase B; positive regulation of peptidyl-serine phosphorylation; peripheral nervous system development; positive regulation of peptidyl-tyrosine phosphorylation; neuromuscular synaptic transmission; nerve development; smooth muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; brain development; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cell migration

0.002 mg

140 USD

0.01 mg

205

1 mg

4550