protein

Recombinant Human Stem Cell Factor

MBS142132

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Stem Cell Factor

Stem Cell Factor

SCF Human; Stem Cell Factor Human Recombinant; Kit ligand Precursor; C-kit ligand; SCF; Mast cell growth factor; MGF; SF; KL-1; Kitl; DKFZp686F2250

kit ligand isoform b; Kit ligand; kit ligand; c-Kit ligand; familial progressive hyperpigmentation 2; mast cell growth factor; steel factor; stem cell factor; KIT ligand; Mast cell growth factor; MGF; Stem cell factor; SCF; c-Kit ligandSoluble KIT ligand; sKITLG

SCF

KITLG; KITLG; SF; MGF; SCF; FPH2; FPHH; KL-1; Kitl; SHEP7; MGF; SCF; MGF; SCF; sKITLG

SCF_HUMAN

E Coli

273

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.

Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

Lyophilized from a concentrated (1mg/ml) solution in water containing 0.02% NaHCO3. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution SCF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Stem Cell Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.52 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Stem Cell Factor as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 500,000 IU/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Stem Cell Factor

Description: Stem Cell Factor Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is purified by proprietary chromatographic techniques. Introduction: Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surface bound SCF by metalloproteases. SCF is a growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and other hematopoietic progenitor cells. One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid).

4505175

NP_000890.1

NM_000899.4

P21583

26,667 Da

Adaptive Immune System Pathway 366160!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 198811!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Disease Pathway 530764!!Downstream Signal Transduction Pathway 106385

This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

0.01 mg

205 USD