Recombinant Human Interleukin-7 | MyBioSource MBS142131 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Interleukin-7

catalog :

MBS142131

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142131

products type :

Recombinant Protein

products full name :

Recombinant Human Interleukin-7

products short name :

Interleukin-7

products name syn :

IL 7 Human; Interleukin-7 Human Recombinant; Lymphopoietin 1 (LP-1); pre-B cell factor; IL-7

other names :

interleukin-7 isoform 1; Interleukin-7; interleukin-7; interleukin 7

products gene name :

IL-7

other gene names :

IL7; IL7; IL-7; IL-7

uniprot entry name :

IL7_HUMAN

host :

E Coli

sequence length :

177

sequence :

MDCDIEGKDG KQYESVLMVS IDQLLDSMKE IGSNCLNNEF NFFKRHICDA NKEGMFLFRA ARKLRQFLKM NSTGDFDLHL LKVSEGTTIL LNCTGQVKGR KPAALGEAQP TKSLEENKSL KEQKKLNDLC FLKRLLQEIK TCWNKILMGT KEH.

purity :

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

Lyophilized from a concentrated (1mg/ml) solution in water containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL7 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Interleukin -7 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.418 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-7 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of murine IXN/2B cells is < 0.5ng/ml, corresponding to a Specific Activity of 2,000,000 IU/mg.

products categories :

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

products description :

Description: Interleukin-7 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 153 amino acids and having a molecular mass of 17412 Dalton. The IL-7 is purified by proprietary chromatographic techniques. Introduction: IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.

ncbi gi num :

4504677

ncbi acc num :

NP_000871.1

ncbi gb acc num :

NM_000880.3

uniprot acc num :

P13232

ncbi mol weight :

7,687 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway 366171!!Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Hematopoietic Cell Lineage Pathway 83078!!Hematopoietic Cell Lineage Pathway 489!!Immune System Pathway 106386!!Interleukin-7 Signaling Pathway 530762!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!PI3K-Akt Signaling Pathway 692234

ncbi summary :

The protein encoded by this gene is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development. This cytokine can be produced locally by intestinal epithelial and epithelial goblet cells, and may serve as a regulatory factor for intestinal mucosal lymphocytes. Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their presence in normal tissues has not been confirmed.[provided by RefSeq, Dec 2010]

size :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage