protein

Recombinant Mouse Glial-Derived Neurotrophic Factor

MBS142120

0.002 mg

140 USD

Native Protein

Recombinant Mouse Glial-Derived Neurotrophic Factor

Glial-Derived Neurotrophic Factor

GDNF Mouse; Glial-Derived Neurotrophic Factor Mouse Recombinant; ATF1; ATF2; HFB1-GDNF; GDNF

glial cell line-derived neurotrophic factor isoform 2; Glial cell line-derived neurotrophic factor; glial cell line-derived neurotrophic factor; ATF; astrocyte-derived trophic factor; mGDNF; glial cell line derived neurotrophic factor; Astrocyte-derived trophic factor; ATF

mGDNF

Gdnf; Gdnf; AI385739; mGDNF; ATF

GDNF_MOUSE

E Coli

214

MSPDKQAAL PRRENRNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI.

Greater than 98.0% as determined by analysis by SDS-PAGE.

GDNF was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Glial-derived Neurotrophic Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GDNF should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Glial Derived Neurotrophic Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: The ED50 as determined by the dose-dependent proliferation of C6 cells, is 0.8-0.12 ug/ml.

CYTOKINES AND GROWTH FACTORS; Neurotrophins; GDNF

Description: Glial derived Neurotrophic Factor Mouse Recombinant produced in E Coli is a non-glycosylated homodimer containing 2 x 135 amino acids and having a total molecular mass of 30.2kDa. GDNF is purified by proprietary chromatographic techniques. Introduction: GDNF promotes the survival and differentiation of dopaminergic neurons in culture, and is able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease.GDNF enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.

672349281

NP_001288261.1

NM_001301332.1

P48540

26,606 Da

Axon Guidance Pathway (1111058); Developmental Biology Pathway (1111057); NCAM Signaling For Neurite Out-growth Pathway (1111067); NCAM1 Interactions Pathway (1111068); Spinal Cord Injury Pathway (739004)

GDNF: Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Defects in GDNF may be a cause of Hirschsprung disease type 3 (HSCR3). In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. Belongs to the TGF-beta family. GDNF subfamily. 5 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region; receptor complex. Molecular Function: glial cell line-derived neurotrophic factor receptor binding; protein homodimerization activity; growth factor activity; transforming growth factor beta receptor binding. Biological Process: peristalsis; enteric nervous system development; neuron differentiation; sympathetic nervous system development; ureteric bud development; transforming growth factor beta receptor signaling pathway; induction of an organ; positive regulation of cell proliferation; response to wounding; postganglionic parasympathetic nervous system development; negative regulation of neuron apoptosis; postsynaptic membrane organization; neural crest cell migration; neurite development; nervous system development; mRNA stabilization; regulation of dopamine uptake; positive regulation of monooxygenase activity; peripheral nervous system development; cell proliferation; ureteric bud branching; regulation of gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; metanephros development; transmembrane receptor protein tyrosine kinase signaling pathway

0.002 mg

140 USD

0.01 mg

205

1 mg

4550