Recombinant Human Growth Hormone Binding Protein | MyBioSource MBS142115 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Growth Hormone Binding Protein

catalog :

MBS142115

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142115

products type :

Recombinant Protein

products full name :

Recombinant Human Growth Hormone Binding Protein

products short name :

Growth Hormone

products name syn :

GHR; GHBP; GH receptor; Somatotropin receptor

other names :

growth hormone receptor isoform 1; Growth hormone receptor; growth hormone receptor; GH receptor; growth hormone binding protein; serum binding protein; somatotropin receptor; growth hormone receptor; Somatotropin receptorGrowth hormone-binding protein; GH-binding protein; GHBPAlternative name(s):Serum-binding protein

products gene name :

GHBP

other gene names :

GHR; GHR; GHBP; GHIP; GH receptor; GH-binding protein; GHBP

uniprot entry name :

GHR_HUMAN

host :

E Coli

sequence length :

638

sequence :

AFSGSEATAAILSRAPWSLQSVNPGLKTNS SKEPKFTKCRSPERETFSCHWTDEVHHGTK NLGPIQLFYTRRNTQEWTQEWKECPDYVSA GENSCYFNSSFTSIWIPYCIKLTSNGGTVD EKCFSVDEIVQPDPPIALNWTLLNVSLTGI HADIQVRWEAPRNADIQKGWMVLEYELQYK EVNETKWKMMDPILTTSVPVYSLKVDKEYE VRVRSKQRNSGNYGEFSEVLYVTLPQMSQF TCEEDFYF

purity :

Greater than 98.0% as determined by: (a) Analysis by SEC-HPLC. (b) Analysis by SDS-PAGE.

form :

Sterile Filtered White lyophilized (freeze-dried) powder. GHBP was lyophilized from a concentrated (1mg/ml) solution with 0.0045mM NaHCO3.

storage stability :

Lyophilized Growth Hormone Binding Protein although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution GHBP should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

other info1 :

Biological Activity: GHBP is fully biologically active as evidenced by its ability of forming 2:1 complex with HGH.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Growth Hormone Binding Protein in sterile 18M?-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of GHBP as a Reference Standard.

products categories :

CYTOKINES AND GROWTH FACTORS; Growth Factors; Growth Hormone

products description :

Description: Growth Hormone Binding Protein Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 248 amino acids and having a molecular mass of 28107.01 Dalton. GHR is purified by proprietary chromatographic techniques. Introduction: GHBP is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. A common alternate allele of this gene, called GHRd3, lacks exon three and has been well-characterized. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. Other splice variants, including one encoding a soluble form of the protein (GHRtr), have been observed but have not been thoroughly characterized.

ncbi gi num :

4503993

ncbi acc num :

NP_000154.1

ncbi gb acc num :

NM_000163.4

uniprot acc num :

P10912

ncbi mol weight :

69,237 Da

ncbi pathways :

Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Endochondral Ossification Pathway (198812); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

ncbi summary :

This gene encodes a member of the type I cytokine receptor family, which is a transmembrane receptor for growth hormone. Binding of growth hormone to the receptor leads to receptor dimerization and the activation of an intra- and intercellular signal transduction pathway leading to growth. Mutations in this gene have been associated with Laron syndrome, also known as the growth hormone insensitivity syndrome (GHIS), a disorder characterized by short stature. In humans and rabbits, but not rodents, growth hormone binding protein (GHBP) is generated by proteolytic cleavage of the extracellular ligand-binding domain from the mature growth hormone receptor protein. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

GH receptor: Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway. Defects in GHR are a cause of Laron syndrome (LARS). A severe form of growth hormone insensitivity characterized by growth impairment, short stature, dysfunctional growth hormone receptor, and failure to generate insulin-like growth factor I in response to growth hormone. Defects in GHR may be a cause of idiopathic short stature autosomal (ISSA). Short stature is defined by a subnormal rate of growth. Belongs to the type I cytokine receptor family. Type 1 subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Receptor, cytokine; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5p13-p12. Cellular Component: extracellular space; cell surface; integral to plasma membrane; integral to membrane; extracellular region; plasma membrane; receptor complex. Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; protein binding; protein homodimerization activity; growth factor binding; peptide hormone binding; protein kinase binding. Biological Process: succinate metabolic process; oxaloacetate metabolic process; activation of MAPK activity; positive regulation of multicellular organism growth; fatty acid metabolic process; tyrosine phosphorylation of JAK2 protein; response to estradiol stimulus; positive regulation of tyrosine phosphorylation of Stat3 protein; 2-oxoglutarate metabolic process; allantoin metabolic process; receptor internalization; creatinine metabolic process; isoleucine metabolic process; cytokine and chemokine mediated signaling pathway; citrate metabolic process; valine metabolic process; regulation of multicellular organism growth; endocytosis; JAK-STAT cascade; creatine metabolic process; multicellular organismal metabolic process; cellular response to hormone stimulus; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of tyrosine phosphorylation of Stat5 protein; insulin-like growth factor receptor signaling pathway; response to cycloheximide; taurine metabolic process. Disease: Hypercholesterolemia, Familial; Laron Syndrome

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3260

more info or order :

MyBioSource product webpage