protein

Recombinant Human Interleukin-15

MBS142107

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Interleukin-15

Interleukin-15

IL 15 Human; Interleukin-15 Human Recombinant; IL-15; MGC9721

interleukin-15 isoform 1 preproprotein; Interleukin-15; interleukin-15; interleukin 15

IL-15

IL15; IL15; IL-15; IL-15

IL15_HUMAN

E Coli

162

MNWVNVISDL KKIEDLIQSM HIDATLYTES DVHPSCKVTA MKCFLLELQV ISLESGDASI HDTVENLIIL ANNSLSSNGN VTESGCKECE ELEEKNIKEF LQSFVHIVQM FINTS.

Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis SDS-PAGE.

The protein was lyophilized from PBS pH 7.4. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IL15 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. Biological Activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be < 0.5 ng/ml, corresponding to a Specific Activity of 2 x 106 IU/mg.

CYTOKINES AND GROWTH FACTORS; Cytokines; Interleukin

Description: Interleukin-15 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 114 amino acids (and an N-terminal Methionine) and having a molecular mass of 12.8kDa. The IL-15 is purified by proprietary chromatographic techniques. Introduction: The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.

10835153

NP_000576.1

NM_000585.4

P40933

14,912 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Cytokines And Inflammatory Response Pathway 198794!!HTLV-I Infection Pathway 373901!!HTLV-I Infection Pathway 373889!!Herpes Simplex Infection Pathway 377873!!Herpes Simplex Infection Pathway 377865!!Intestinal Immune Network For IgA Production Pathway 128760!!Intestinal Immune Network For IgA Production Pathway 128670!!Jak-STAT Signaling Pathway 83077

The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]

0.01 mg

205 USD