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Recombinant Mouse Insulin Like Growth Factor-1

MBS142106

0.01 mg

140 USD

MBS142106

Recombinant Protein

Recombinant Mouse Insulin Like Growth Factor-1

Insulin Like Growth Factor-1

IGF1 Mouse; Insulin-Like Growth Factor 1 Mouse Recombinant; Somatomedin C; IGF-I; IGFIA; IGF1

insulin-like growth factor I isoform 4 preproprotein; Insulin-like growth factor I; insulin-like growth factor I; somatomedin; insulin-like growth factor 1; Somatomedin

mIGF1

Igf1; Igf1; Igf-1; Igf-I; C730016P09Rik; Igf-1; IGF-I

IGF1_MOUSE

E Coli

153

The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu.

Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein was lyophilized with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized IGFI although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution IGF-1 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized IGF1 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IGF-I as a Reference Standard. Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is < 1.0 ng/ml, corresponding to a specific activity of 1MU/mg.

CYTOKINES AND GROWTH FACTORS; Growth Factors; Insulin-Like Growth Factor

Description: Insulin-Like Growth Factor I mouse Recombinant produced in E Coli is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7600 Dalton. IGF-I is purified by proprietary chromatographic techniques. Introduction: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early studies showed that growth hormone did not directly stimulate the incorporation of sulfate into cartilage, but rather acted through a serum factor, termed 'sulfation factor,' which later became known as 'somatomedin' (Daughaday et al., 1972). Three main somatomedins have been characterized: somatomedin C (IGF1), somatomedin A (IGF2; MIM 147470), and somatomedin B (MIM 193190) (Rotwein, 1986; Rosenfeld, 2003).

163659894

NP_001104745.1

NM_001111275.1

P05017

17,623 Da

AMPK Signaling Pathway 989651!!AMPK Signaling Pathway 992181!!Adipogenesis Pathway 198299!!Aldosterone-regulated Sodium Reabsorption Pathway 130627!!Aldosterone-regulated Sodium Reabsorption Pathway 130590!!Androgen Receptor Signaling Pathway 198319!!Apoptosis Pathway 198339!!Dilated Cardiomyopathy Pathway 121495!!Dilated Cardiomyopathy Pathway 121285!!Endochondral Ossification Pathway 198336

0.01 mg

140 USD