protein

Recombinant Human Tumor Necrosis Factor-beta

MBS142101

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Tumor Necrosis Factor-beta

Tumor Necrosis Factor-beta

TNF b Human; Tumor Necrosis Factor-Beta Human Recombinant; Lymphotoxin-alpha; LT-alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1; LTA; LT; TNFB; TNFSF1

lymphotoxin-alpha; Lymphotoxin-alpha; lymphotoxin-alpha; LT-alpha; TNF superfamily, member 1; TNF-beta; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1; lymphotoxin alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1

TNF b

LTA; LTA; LT; TNFB; TNFSF1; TNFB; TNFSF1; LT-alpha

TNFB_HUMAN

E Coli

205

MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAG.

Lyophilized protein with no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Tumor Necrosis Factor-b although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TNF-b should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-beta in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TNF-b as a Reference Standard. Biological Activity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.

CYTOKINES AND GROWTH FACTORS; Cytokines; Tumor Necrosis Factor

Description: Tumor Necrosis Factor-b Human Recombinant (Lymphotoxin) produced in E Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 18645 Dalton. The TNF-b is purified by standard chromatographic techniques. Introduction: Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.

6806893

NP_000586.2

NM_000595.3

P01374

22,297 Da

Apoptosis Pathway (198797); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); IL2 Signaling Events Mediated By STAT5 Pathway (137988); IL4-mediated Signaling Events Pathway (137933); NF-kappa B Signaling Pathway (634527)

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family. Protein type: Apoptosis; Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: extracellular space; membrane. Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding. Biological Process: response to drug; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of apoptosis; apoptosis; response to lipopolysaccharide; signal transduction; lymph node development; humoral immune response; positive regulation of interferon-gamma production; defense response to Gram-positive bacterium; cell-cell signaling; negative regulation of fibroblast proliferation; response to hypoxia; positive regulation of chronic inflammatory response to antigenic stimulus; response to nutrient. Disease: Psoriatic Arthritis, Susceptibility To; Myocardial Infarction, Susceptibility To; Leprosy, Susceptibility To, 4

0.005 mg

140 USD

0.02 mg

205

1 mg

2665